FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE6-EV-RFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE6-EV-RFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12428702
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT240030.19312555727862812No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC215160.17311542267245608No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA158490.1275193499691279No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC157140.12643315448387127No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG152400.12261940144674803No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG148060.11912748410896006No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC131330.10566670598426127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG103350.027.9777325
GCTGACT66500.026.163671
CTAGTTG46800.024.5293088
TACTCTA51700.022.4120774
GACTCTA83550.021.9966984
CTAGCAG82400.021.8319858
GGACACC50350.021.27066429
TCTAGTT58600.020.6057057
CTGACTC86850.020.314712
CTAATGC55200.020.22627421
ACCTCTA55350.020.04475217
TGTACTC58500.019.627632
GACACCT55100.019.37346630
AGGACTG59200.019.33229362
TTGTACT58950.019.2408891
CTCTAGT62900.019.0334876
GTACTCT62150.018.9253033
CTCTAGC98700.018.513836
GTTACCT60550.018.49672714
TAGTTGT65300.018.276739
Produced by FastQC (version 0.11.5)