FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE5-SCL15-GFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE5-SCL15-GFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12163393
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT225250.18518681423842837No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT171540.1410297274781798No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC158330.13016927102495165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT61250.027.2621861
ACTCTAG86700.026.6478735
CTAGCAG73050.022.9022188
GACTCTA74600.022.5706444
CTGACTC78150.020.9633142
CTCTAGC91500.018.4020086
CTATCGA89700.018.10427317
TACTCTA38350.017.7081854
CTAGTTG38050.017.5690618
TAGCAGA94750.017.546039
TCTATCG93950.017.47155816
TGACTCT103000.016.4151863
AGGACTG45950.016.29985862
GATCTAT103300.016.02574214
GGACACC39450.015.96918829
CTAGCAT102900.015.74791126
TAGTAGG32250.015.73604558
GGACTGT47900.015.56335463
TCTAGTT48650.015.3239947
GACACCT41850.015.30428230
Produced by FastQC (version 0.11.5)