FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE4-bZIP63-RFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE4-bZIP63-RFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12664565
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT251280.19841186807442657No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC222030.17531593070902948No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA178250.1407470371070779No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG164560.12993734881537583No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG150740.11902501191316085No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC145960.11525070146507205No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC133720.10558593998293664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG108050.030.4530335
GCTGACT69900.027.6946561
TACTCTA52950.025.8487784
CTAGTTG53350.025.5854178
CTAGCAG80250.024.1616748
GACTCTA81650.024.0940234
TGTACTC59150.023.1988072
GGACACC54050.023.18165829
GTACTCT60150.022.870963
TCTAGTT60700.022.8339397
CTGACTC85900.022.6169572
CTCTAGT65050.022.0092456
ACCTCTA62650.021.73112917
GACACCT59750.021.55594630
TTGTACT63100.021.4698811
CTAATGC62200.021.32625421
CTCTAGC98700.020.0028176
CACCTTA47300.019.8311547
AGGACTG71450.019.69159762
TAGTTGT71000.019.6686769
Produced by FastQC (version 0.11.5)