FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE30-XERIR0-GFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE30-XERIR0-GFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10049987
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG63550.024.6767585
GCTGACT36450.022.1854461
TACTCTA34950.021.6336634
GGACACC34850.020.28650529
CTAGTTG38300.020.012928
CTAGCAG42900.019.090788
GCACACG13650.018.46132311
AGGACTG42400.018.32498462
GACACCT38850.018.287830
GGACTGT43750.017.75952763
TCTAGTT47400.017.5742457
CTAATGC42200.017.33400521
ACCTCTA43450.017.31838217
GTACTCT44750.017.2870373
CTGACTC47500.017.0237332
GACTCTA48450.016.9061834
ATGCTGG44250.016.84742724
GTTACCT45950.016.29989214
TACCTCT48350.015.346116
TAGTAGG30350.015.337316558
Produced by FastQC (version 0.11.5)