FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE3-XERIR0-GFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE3-XERIR0-GFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10082721
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT198400.19677228002242647No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT149800.14857100578306193No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC133060.1319683446561697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT51450.027.89631
ACTCTAG72150.027.1215785
CTAGCAG61650.023.6167458
GACTCTA65100.023.336944
CTGACTC66000.021.9582672
CTCTAGC80450.019.1454756
TAGCAGA79650.019.070489
CTATCGA78300.018.37120617
TCTATCG80500.017.99939216
TGACTCT87850.017.1341233
ATCTATC91750.015.94496515
GATCTAT91800.015.89815414
CTAGCAT92150.015.61012226
TTCTAGC94700.015.33769424
GGACAGC92600.015.2324240
CTAGTTG30100.015.2322688
GCAGATC97550.015.1764711
AGATCTA98150.014.97656913
TACTCTA30300.014.9032944
GGACACC27250.014.77028829
Produced by FastQC (version 0.11.5)