FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE29-SCL15-GFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE29-SCL15-GFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13424598
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG65550.018.744985
GCTGACT48550.016.8729781
CTAGCAG46800.016.6771648
GACTCTA52400.016.367494
GGACACC32150.013.28129529
CTAGTTG35700.013.0390718
CTAGCAT60000.012.83323826
CTATCGA62600.012.57949717
GGACAGC58950.012.46814640
CTGACTC65100.012.3143512
TCTATCG64200.012.04792716
TACTCTA39250.011.5944734
GACACCT37700.011.41888530
TCTAGCA97450.011.3856227
GCCCCCT5955.4569682E-1111.1789681
CGGTCGG65750.011.12514451
AGGACTG40850.011.05238562
GCACACG16200.011.01827211
TCGGTCG67250.010.98108550
GGACTGT43350.010.81871463
Produced by FastQC (version 0.11.5)