FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE28-bZIP63-RFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE28-bZIP63-RFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13894550
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT229850.16542457294406798No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC175330.12618616651852704No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA171510.12343688712480794No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG149010.1072434875544728No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG141440.10179530823236449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT63650.026.5648441
ACTCTAG93250.024.36315
CTAGCAG74800.023.0679268
GACTCTA81200.021.8568234
CTGACTC80550.021.2081072
GCACACG18450.018.4006811
TAGCAGA100250.017.5257999
CTATCGA99750.017.19248217
TCTATCG99500.017.05980516
TGACTCT102800.016.9578723
CTCTAGC107950.016.4408236
GGACACC32950.016.0391629
CTAGTTG37200.015.9944468
ACTCCCG21400.015.70059132
TAGCATG111900.015.35718127
TACTCTA38750.015.2668744
GATCTAT113550.015.10303914
CTAGCAT116900.014.67044426
GCAGATC122100.014.5901811
ATCTATC119000.014.3230615
Produced by FastQC (version 0.11.5)