FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE27-AL7-RFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE27-AL7-RFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12004982
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT180060.14998773009405594No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC129010.10746371797975207No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA124720.10389020158464211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG84250.027.007025
GCTGACT53000.025.2311461
CTAGCAG57050.023.6806898
TACTCTA43550.021.8631214
CTAGTTG44200.021.8549338
GACTCTA65300.021.4962444
GGACACC44250.020.40635729
CTGACTC69050.019.2644562
ACCTCTA49750.019.13512217
GACACCT47850.019.0172930
AGGACTG54350.018.6104762
TCTAGTT54800.018.3943167
GGACTGT56850.018.03847363
GTACTCT53800.017.8278793
CTATCGA75100.017.70920617
CTAATGC52650.017.54956821
CTCTAGT55900.017.4087686
TACCTCT55900.017.21774916
CTCTAGC83000.017.1652986
TCTATCG77700.017.16166516
Produced by FastQC (version 0.11.5)