FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE26-EV-RFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE26-EV-RFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13182839
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT167630.12715773893620336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG88250.027.2113785
GCTGACT58200.024.7216851
CTAGCAG65700.021.9479488
GACTCTA69950.021.3688564
CTAGTTG47100.021.0294348
TACTCTA47750.020.2337724
CTGACTC72100.020.0037462
GGACACC45300.019.9333529
GACACCT52100.017.86916730
CTATCGA80300.017.78293217
ACCTCTA53350.017.7784417
TCTAGTT59300.017.7065337
TAGCAGA85300.017.4381479
AGGACTG59150.017.21856962
TCTATCG81800.017.20012316
CTCTAGC89350.016.6508796
CTCTAGT61900.016.4002386
GGACTGT61500.016.38989463
GTTACCT61500.015.87776914
CTAATGC57850.015.85106121
Produced by FastQC (version 0.11.5)