FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE25-XERIR0-GFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE25-XERIR0-GFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12446184
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA146610.11779514106492399No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA137290.1103069020994708No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT128370.1031400467806036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG93950.031.5972545
GCACACG25050.031.01723111
TACTCTA68150.029.9469744
CTAGTTG73100.027.2910658
GTACTCT76950.026.7951973
TCTAGTT81700.025.361197
ACGTCTG31500.025.11031515
CTCTAGT84350.024.4031546
GGACACC80200.023.9584829
GACACCT82250.023.36124430
AGCACAC35050.023.06650210
ACCTCTA86900.022.7553217
ATCGGGG12000.022.45852340
GCTGACT45000.022.1722891
CTAATGC89000.021.98253821
CACGTCT37150.021.85664214
GTTACCT91250.021.78561214
ATGCTGG91200.021.33721524
CACACGT37150.021.19734612
ACACGTC37950.020.93477813
Produced by FastQC (version 0.11.5)