FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE24-SCL15-GFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE24-SCL15-GFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13930502
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA227140.16305227191381902No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT212470.1525214238510572No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA204820.14702987731526115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA96650.033.3939674
ACTCTAG120950.031.0555325
GTACTCT105850.030.689943
CTAGTTG112400.028.585158
TCTAGTT119200.027.865187
CTCTAGT119600.027.3670946
GGACACC119600.025.45945229
ACCTCTA127450.024.7422817
CTAATGC127150.024.71834621
GTTACCT132100.024.1098714
GACACCT127450.024.0283830
TGTACTC134950.023.8652692
TTGTACT134550.023.8330021
TACCTCT134550.023.6707716
ATGCTGG133150.023.39453924
TGGACAC133150.022.97371528
TGTTACC140550.022.88455813
TTACCTC140400.022.73434415
AGGACTG151850.022.35682562
GGACTGT152950.022.26476363
Produced by FastQC (version 0.11.5)