FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE23-bZIP63-RFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE23-bZIP63-RFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14851276
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT198660.1337662837859858No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC155590.10476540870966239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG97700.025.2957865
GCTGACT63700.023.7960831
CTAGCAG72150.021.1500878
TACTCTA50850.020.2391824
GACTCTA77250.019.9384214
CTAGTTG53200.019.078688
CTGACTC80150.018.6058042
GGACACC50700.018.017529
TCTAGTT64900.017.0958127
ACCTCTA56300.017.09550317
CTATCGA90700.016.47700117
AGGACTG64450.016.23694862
GTACTCT63100.016.143543
GACACCT57550.016.11614230
TCTATCG91350.016.0914616
GGACTGT66350.015.93028663
CTCTAGC101250.015.7655036
CTCTAGT66650.015.7039566
CTAATGC59950.015.64620721
TAGCAGA98750.015.2757369
Produced by FastQC (version 0.11.5)