FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE22-AL7-RFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE22-AL7-RFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12045673
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG70900.022.4151675
CTAGTTG43150.019.2232138
TACTCTA43500.018.8303764
GGACACC41750.018.44255429
GCTGACT40800.018.275981
ACCTCTA48800.016.6387717
GACACCT48200.016.19245530
CTAGCAG47400.016.0228548
AGGACTG53150.016.00146362
TCTAGTT56450.015.8102367
CTCTAGT55300.015.7618286
GGACTGT55500.015.07186563
GTTACCT54400.014.99041614
TAGTAGG38200.014.75092258
CTAATGC53000.014.66029721
GTACTCT55250.014.63565353
ATGCTGG55300.014.43030124
TGGACAC55150.014.21534428
CTGACTC53800.014.1845512
TACCTCT56400.014.14850716
Produced by FastQC (version 0.11.5)