FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE21-EV-RFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE21-EV-RFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12888725
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG74000.020.152135
GCTGACT49300.019.2434231
CGTATGC23800.018.38246344
CCTATAC24050.017.75450933
GCACACG24950.017.67497611
GTATGCC25700.017.56806245
TATGCCG26550.016.34623346
ACGTCTG28700.015.97502315
CTAGCAG59600.015.6206568
ACCTATA27550.015.49876532
GACTCTA64000.015.3696644
CTGACTC63150.014.8008872
CACCTAT30100.014.30196731
TACTCTA40050.014.1595864
CTCGTAT30150.014.04657442
GGACACC35250.013.99997529
CTAGTTG39200.013.6605848
CTATCGA67850.013.56624617
CACACGT33450.013.49731412
AGCACAC34800.013.37620510
Produced by FastQC (version 0.11.5)