FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE20-XERIR0-GFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE20-XERIR0-GFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11252611
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT39500.016.9278161
ACTCTAG50500.016.150965
CTAGCAG42500.016.0585448
GACTCTA50200.014.8528474
CTGACTC51950.013.2071822
GCACACG13700.012.77355711
CTAGCAT50850.012.66436726
CTATCGA53000.012.41476717
GCCGGGG14050.012.2091231
GGACAGC51800.012.16243740
TCTAGCA84600.011.7908387
TCTATCG54700.011.70896316
GTCCGCT9100.011.1563291
CTCTAGC67600.010.9263566
GGTGGAC61350.010.66839537
TAGCAGA66750.010.6440569
TTCTAGC62650.010.50251724
GACAGCA63050.010.32537341
CGCCCCT6251.5024852E-910.0822431
GCCCCAT13250.010.039971
Produced by FastQC (version 0.11.5)