FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE2-AL7-RFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE2-AL7-RFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9754405
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT200150.2051893477869742No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC182970.1875767922287418No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG150430.15421750480936564No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA139640.14315583574805432No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC133640.13700476861479507No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG121570.12463087189838848No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG114120.11699329687459153No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC112880.11572207633371795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT53100.027.8204731
ACTCTAG71250.027.4640545
GCACACG17100.025.78924611
GACTCTA64700.023.5350974
GGCCTTA18350.023.46040536
CTAGCAG65200.023.24398
GTATGCC17250.023.12975747
TATGCCG17050.022.7851448
CGTATGC17500.022.59980246
CTGACTC68950.021.4248662
ACGTCTG21400.021.09740615
GTGGCCT21050.020.45102334
CACACGT22050.020.15824312
ACACGTC22400.019.84306713
CTCTAGC78050.019.5997586
AGCACAC23450.019.2537610
GTCACGT22700.019.11857829
AGTCACG24250.018.47387328
TGACTCT82150.018.4504473
CTATCGA82150.018.44723117
Produced by FastQC (version 0.11.5)