FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE19-SCL15-GFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE19-SCL15-GFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10356297
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT34450.017.5797161
ACTCTAG43950.016.8852235
CTAGCAG37850.015.9970858
GACTCTA44300.014.5393144
GCCGGGG12700.013.50663951
GGACAGC42250.013.42021340
CTATCGA46250.012.86470617
CTAGCAT43800.012.78510926
GCCCCCT5551.4551915E-1111.9843821
CTGACTC49850.011.9376782
TCTATCG48500.011.8348516
CGGGGAT17850.011.5709441
CGGTCGG48550.011.31803551
TAGCAGA56600.011.2542259
CTCTAGC59400.011.1968786
GCGGGCT6703.6379788E-1210.9723451
CGGGAAT61600.010.9681041
TCGGTCG50900.010.93301550
TCTAGCA74850.010.8484397
GTCGGGA54000.010.62944753
Produced by FastQC (version 0.11.5)