FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE18-bZIP63-RFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE18-bZIP63-RFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9458948
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT136490.1442972305165437No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC105820.11187290595106349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG66250.024.5168195
GCTGACT41750.024.1484181
CTAGCAG46950.021.6181558
GACTCTA51800.020.5434574
CTGACTC52800.018.9616262
TACTCTA31850.018.5740664
GGACACC31850.017.58221829
CTAGTTG34400.017.19438
TCTATCG61350.016.82920616
CTATCGA62600.016.77270717
ACCTCTA34750.016.41681917
GACACCT35150.015.93145730
GCACACG13100.015.76293111
CTCTAGC71900.015.7255426
TAGCAGA67600.015.480359
TCTAGTT42700.015.0818777
TGACTCT71600.015.0091143
TAGCATG66850.014.97373227
GTTACCT40150.014.90619514
GTACTCT40250.014.5238013
Produced by FastQC (version 0.11.5)