FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE17-AL7-RFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE17-AL7-RFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10581733
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT154090.1456188698013832No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC113150.10692955492261995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG76300.027.0691385
GCTGACT48600.023.9866331
TACTCTA39050.022.6800694
CTAGCAG57300.021.195218
GACTCTA60150.020.8349444
CTAGTTG41350.020.2295048
GGACACC40700.019.69245329
CTGACTC62250.019.0637052
GTACTCT47500.018.3506163
CTCTAGT49900.018.0294136
ACCTCTA47600.017.86709617
AGGACTG50800.017.77504262
TCTAGTT49000.017.7145757
GACACCT46300.017.53733830
GGACTGT52150.017.31514563
GTTACCT48300.017.17362614
CTCTAGC74900.016.965786
TAGTAGG37650.016.91846758
TGTACTC51350.016.9066752
TCTATCG70200.016.90126616
Produced by FastQC (version 0.11.5)