FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE16-EV-RFP-Shoot.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE16-EV-RFP-Shoot.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10834231
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT132490.12228832853942288No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC112010.1033852794905333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG86700.027.5752455
TACTCTA50350.025.3057774
GCTGACT49150.023.2183781
CTAGTTG55700.022.9977598
GGACACC54650.022.47908229
CTCTAGT59750.022.0862086
TCTAGTT60600.021.889627
TGTACTC57900.021.7647512
ACCTCTA60500.020.65239717
TTGTACT61400.020.2394081
GTTACCT62800.020.06321314
CTAGCAG57450.019.9822088
CTGACTC58550.019.7295342
GTACTCT63700.019.7275313
GACACCT62550.019.63990830
TAGTTGT66950.019.6037129
TACCTCT64050.019.5077316
CTAATGC64200.019.40799321
CACCTTA42050.018.8110967
TGGACAC65600.018.7802128
Produced by FastQC (version 0.11.5)