FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE15-SCL15-GFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE15-SCL15-GFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12322997
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT224200.1819362611221929No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT173440.14074498273431374No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC142840.11591336101112416No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA129190.10483651014440724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG107750.029.465585
GCTGACT64700.027.0525591
CTAGTTG59950.024.11138
TACTCTA60350.023.8389844
CTAGCAG74700.023.7545688
GACTCTA76950.023.290814
GGACACC59700.022.51222629
TCTAGTT66550.022.2462797
GTACTCT64850.022.2387563
CCAGGCG17050.021.75920533
CTGACTC82100.021.4888972
ACCTCTA66600.021.17810417
CTCTAGT71100.020.9240656
GACACCT64400.020.86908330
GCACACG18900.020.7404311
CTAATGC67000.020.73849721
TACCTCT72500.019.35817316
AGGACTG78950.019.06241262
GTTACCT73950.018.9786814
ATGCTGG73450.018.96515324
Produced by FastQC (version 0.11.5)