FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE14-bZIP63-RFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE14-bZIP63-RFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10215053
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT160440.15706232752781607No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC143090.1400775894163251No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA140780.1378162208262649No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA135200.13235369410222345No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA132080.12929937808447983No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG112950.11057211352696848No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT107640.10537390261215482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA58500.030.7574354
ACTCTAG100900.030.4958345
GTACTCT61750.029.3086933
CTAGTTG64250.027.9993428
TCTAGTT67750.027.5868767
TGTACTC66100.027.2212942
TTGTACT65300.027.1804561
CTAATGC69850.024.90289921
GCTGACT49950.024.529831
TAGTTGT75000.023.9861019
GTTACCT74650.023.67648514
CTCTAGT79450.023.5724586
ATGCTGG74600.023.31725924
TGGACAC74200.023.20728
TACCTCT76900.023.12039216
ACCTCTA77500.023.03171717
TTACCTC78200.022.87019215
CTAGCAG55500.022.32388
TCTAATG78800.022.11876920
GGACTGT88050.022.1005663
Produced by FastQC (version 0.11.5)