FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE13-XERIR0-GFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE13-XERIR0-GFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12717698
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT227120.178585778652709No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT196200.1542732025874494No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC155440.12222337721810976No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA145660.11453330626344485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG107150.032.1802945
GCTGACT62650.028.3313311
TACTCTA62650.027.3792114
CTAGTTG64750.025.837198
GTACTCT67450.025.3270053
GGACACC65750.024.32638729
GACTCTA74600.024.2603844
CTAGCAG74250.024.0398038
TCTAGTT72950.023.6046167
CTCTAGT75050.023.0888426
ACCTCTA72350.022.92944517
CTAATGC72650.022.7387721
GACACCT70450.022.5543530
CTGACTC80950.022.0982842
ATGCTGG76650.021.41532524
TGGACAC78750.020.62170628
GTTACCT80750.020.50095414
TACCTCT81950.020.4996316
TTACCTC84100.019.97556115
AGGACTG89150.019.90415662
Produced by FastQC (version 0.11.5)