FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE12-AL7-RFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE12-AL7-RFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13899658
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT295000.2122354377352306No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC217110.1561980877515116No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA197590.14215457675289564No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG186690.13431265718911933No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC163230.11743454407295488No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG156420.11253514295099921No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG140490.10107442931329678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG135150.030.2007965
GCTGACT80350.029.0159451
TACTCTA63500.026.956874
CTAGTTG68550.024.864928
CTAGCAG97850.024.5731878
GACTCTA99200.024.3484754
TCTAGTT74200.024.0567267
GTACTCT72650.023.3208333
GGACACC70550.023.16750129
CTGACTC100800.023.128782
TGTACTC73700.023.0838262
CTCTAGT77650.022.9464426
CTAATGC72600.022.85094621
TTGTACT73750.022.736411
ACCTCTA75850.022.10231217
GACACCT74650.022.03564630
GTTACCT80000.020.7370114
AGGACTG89450.020.6981562
ATGCTGG81050.020.51176624
GGACTGT90850.020.4949163
Produced by FastQC (version 0.11.5)