FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE11-EV-RFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE11-EV-RFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13243244
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT249670.18852631575767992No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC212030.1601042765654699No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA170490.12873733958235611No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG163000.12308162561982547No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG158500.1196836666303211No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC148210.11191366707432107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG114100.027.9484865
GCTGACT74800.026.0215551
TACTCTA51050.024.7534314
CTAGTTG53150.023.5085898
GGACACC51500.022.76656729
CTAGCAG89450.021.911368
GACTCTA93150.021.4574054
TCTAGTT62700.020.598177
CTGACTC94000.020.5935422
TGTACTC60300.020.4923232
GACACCT58400.020.31632830
GTACTCT64400.019.5677383
ACCTCTA61000.019.56521217
CTCTAGT66650.019.5374726
CTAATGC62300.019.49424221
AGGACTG65850.019.39979262
GGACTGT67550.018.80794163
CTCTAGC109550.018.46896
GTTACCT66600.018.39298414
CTATCGA106050.018.34949317
Produced by FastQC (version 0.11.5)