FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE10-SCL15-GFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE10-SCL15-GFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11484658
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT199410.17363163970577095No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT147940.12881532911123691No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC135070.11760907464549662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG98700.030.5356865
TACTCTA54400.026.7680134
GCTGACT59200.026.550741
CGTATGC20000.025.89959344
GCACACG21450.025.7804811
CTAGTTG56100.025.5786978
TATGCCG20000.025.37460146
GTATGCC21050.024.94022645
GTACTCT58550.024.8707083
ACCTCTA60850.023.23690217
TCTAGTT64850.023.2074417
GGACACC58350.023.1530229
CTCTAGT64500.023.1192366
CTAGCAG68500.022.9410468
GACTCTA73150.022.347264
CTAATGC62850.022.21941421
GACACCT60950.022.16516930
CACACGT24850.021.689812
CTGACTC73750.021.4062232
ACACGTC24950.021.1819413
Produced by FastQC (version 0.11.5)