FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_GE1-EV-RFP-Root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_GE1-EV-RFP-Root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10482200
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT178300.17009788021598518No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC162690.15520596821277977No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA134310.12813149911278168No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG132520.12642384232317644No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG116600.11123619087596115No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC113010.10781133731468585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG82850.027.3800335
GCTGACT56150.024.5020581
TACTCTA38700.022.7949684
CTAGCAG65450.021.8714758
GACTCTA66500.021.6882724
CTAGTTG40400.021.0518058
CTGACTC68050.020.4739822
GGACACC42550.019.16525529
GTACTCT47450.018.6650683
TGTACTC48050.018.3592322
CTAATGC44700.018.32182121
CTCTAGC81350.018.116666
CTATCGA78050.018.02649917
GACACCT46150.017.89758530
CTCTAGT51750.017.7232956
TCTAGTT49950.017.6580187
TTGTACT48500.017.5397871
TCTATCG79150.017.5106616
TAGCAGA83350.017.3423889
ACCTCTA49950.017.23684917
Produced by FastQC (version 0.11.5)