FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_ChIP.zmm16-line15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_ChIP.zmm16-line15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9679128
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1467471.5161179808759633TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC181900.064.72623446
TCTCGTA169600.064.449443
GTATGCC183900.063.75586347
ACAATCT170750.063.1967239
AGTCAAC195550.063.07243734
TCACAGT196300.062.91995630
AACAATC174400.062.7969538
ATCTCGT172700.062.4825142
ACAGTCA199200.062.1441932
CACAGTC199000.062.0659531
GCTTGAA176250.061.3002661
CTTGAAA178300.060.98804562
TGCTTGA172200.060.87181560
TATGCCG196550.059.4563448
CGTCTGA212150.058.8622216
TCTGAAC212550.058.6370918
AATCTCG186750.057.8194641
CAGTCAA214000.057.7809533
ACACGTC217500.057.36606613
GCCGTCT194900.057.30251
Produced by FastQC (version 0.11.5)