FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_ChIP.zmm16-line14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_ChIP.zmm16-line14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9375782
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1439181.5349972940923753TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC356950.3807149099669766TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC218250.066.1032844
TCTCGTA170000.066.0681941
GTATGCC220500.066.0001945
AGTCACC275100.065.7243328
TCTGAAC281500.065.04996518
CGTCTGA282450.064.8807416
CTTGTAA275600.064.8547934
TGTAATC198450.064.4608936
ATCTCGT179300.064.2029840
ACACGTC286450.064.0239613
CACACGT287400.063.71456512
GCTTGAA193600.063.2006459
CTTGAAA196350.063.1888960
CTGAACT290750.063.04063419
AATCTCG186450.062.66074439
TAATGTC54700.062.6408638
GCACACG294100.062.3827311
AATGTCG54550.062.2369939
TGCTTGA188350.062.19357358
AGCACAC295650.062.16254810
Produced by FastQC (version 0.11.5)