FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_ChIP.zmm16-line14-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_ChIP.zmm16-line14-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11217146
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC209850.18707967249423338TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT40500.046.0601829
AGTCACG41850.044.2398528
CGTATGC40950.043.8452744
TCACGTA43850.043.25927730
ATGCCGT42950.041.72171847
ACGTCTG45550.041.49146715
TATGCCG44350.040.40486546
CGTAGAG46800.040.159833
CACGTAG47650.039.73615631
ACACGTC47700.039.6213113
ACGTAGA47900.039.30956632
CACGTCT49200.038.62674714
CTCGTAT44100.038.0162942
CGTCTGA51850.036.31491516
TCGTATG49400.036.2045343
CACACGT53250.035.62337512
GCACACG54200.035.19317211
TCTCGTA51050.032.7038841
ACTCCAG57150.032.45850823
CAGTCAC57600.032.26511427
Produced by FastQC (version 0.11.5)