FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_ChIP.zmm16-line12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_ChIP.zmm16-line12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9743613
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC3708413.8059906525433638TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAGCTCGTATGC140660.14436123437989584TruSeq Adapter, Index 11 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA416700.067.0607941
CGTATGC451000.066.9027344
GTATGCC453300.066.6555945
GCTACAT444850.066.6317635
GCTTGAA417850.066.0784559
ATCTCGT417500.066.0691540
CTACATC429500.065.9739236
TCTGAAC484200.065.9003518
CTTGAAA421950.065.8753260
CGTCTGA484650.065.7879316
CACGGCT482500.065.5743331
TGCTTGA412100.065.4207658
CATCTCG422250.065.40848539
ACACGTC487700.065.3761713
CACACGT488600.065.2629112
ACATCTC426150.064.9166538
CCGTCTT440700.064.5348550
GCACACG494550.064.48479511
CTGAACT495500.064.405219
CTCCAGT493250.064.1306724
Produced by FastQC (version 0.11.5)