FastQCFastQC Report
Sat 15 Feb 2020
HCTCMBGXF_n01_ChIP.zmm16-line12-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCTCMBGXF_n01_ChIP.zmm16-line12-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12297321
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT251400.20443477079276048TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGT57050.048.4046735
ACGTCTG60300.045.96880315
ACACGTC61600.044.714613
CGTATCT48350.044.30246739
CGTATGC44850.043.6219146
TTCCGTA62450.043.60267336
CACGTCT64600.042.74642614
AGTTCCG66100.041.93557734
CCGTATC59550.041.61222538
CACACGT66700.041.40075712
GCACACG67200.041.30137611
CGTCTGA68950.040.50642816
GTCACAG68650.040.4798429
TCCGTAT65050.040.40734537
TCACAGT69200.040.20852330
ATGCCGT48650.040.0707749
TATGCCG52150.039.59615748
ACAGTTC71850.038.7253832
ACTCCAG73300.038.05528623
CAGTCAC75050.037.3543127
Produced by FastQC (version 0.11.5)