FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n02 wt - biotin-dc.34200000008932.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n02 wt - biotin-dc.34200000008932.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13213721
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC17350.023.27537744
GTCGCCG18600.022.28440745
GCACACG16150.019.86784218
TGGTCGC23800.016.50162743
CGGGGAC11600.015.6884351
GTGGTCG25750.015.58634642
GCGCCCC11650.014.17382631
CGCGGAC5950.013.8592171
CTCGGTG31950.013.70373544
GAGCACA30400.013.19348916
GCGGGTG14450.013.1845271
CGGCGTA7200.013.032841
AGCACAC25400.012.98340917
GGGCGTC12400.012.84174251
GATCTCG62200.012.26827345
AGAGCAC33750.012.14815
ACACGAG14050.012.13598420
CGTGGAC13950.012.0263941
TCTCGGT37700.011.86637537
ATCTCGG39750.011.77961642
Produced by FastQC (version 0.11.2)