FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n02 rnh201-frbrad27-frb pnk.342000000088ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n02 rnh201-frbrad27-frb pnk.342000000088ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11031084
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC22250.031.08421344
GTCGCCG22950.030.79134445
GCACACG28950.029.54178218
TCAGACG53800.026.8419612
CAGACGT45250.026.70588113
CGTGTGC36650.026.66878717
GTGACTG35700.026.1308081
TCCGATC41300.026.1077627
GACTGGA42500.025.9978123
AGCACAC37500.025.05726417
GAGCACA46350.024.82587416
ACGTGTG39600.024.62600116
TGACTGG39150.024.3932322
AGAGCAC46500.024.22030315
CCGATCT41950.023.95414528
GGAGTTC67550.023.7647637
AGACGTG46700.023.68846914
CTTCCGA51750.023.4515925
TTCAGAC58000.023.41060411
AGTTCAG63150.023.4070689
Produced by FastQC (version 0.11.2)