FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n02 rnh201-frb pnk.3420000000885d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n02 rnh201-frb pnk.3420000000885d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11072309
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG13800.020.9917718
CGGGGAC11000.018.9342041
CGGGGTG8850.018.3758931
CGGGGAG11000.017.637341
GGGGACC15200.016.2908692
GGTCGCC9300.015.51415344
GGCGGTC7600.015.391721
AGCACAC20500.015.32674817
AGGGGAC15250.014.7799881
AGAGCAC27300.014.6108415
GAGCACA27600.014.45202616
GCGGGTC6950.014.3681251
TGGGGTT35100.014.22485351
GGGGTAC16100.013.9977082
GGGGGAT22800.013.8900881
GGGGTAA25600.013.802622
CACACGA14300.013.75768519
TGGGGTA31300.013.6731
CGCGGAG6300.013.5861861
GCGCCCC4650.013.43304231
Produced by FastQC (version 0.11.2)