FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n02 rnh201-frb rad27-frb dna2-frb pnk.34200000008925.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n02 rnh201-frb rad27-frb dna2-frb pnk.34200000008925.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15721949
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC40300.031.29024944
GTGACTG61300.030.0794811
GTCGCCG43100.029.55578245
GACTGGA70200.029.4019643
CAGACGT77200.028.4924113
TGACTGG65400.027.8047832
TCAGACG98700.027.38144512
GGAGTTC116400.026.8919667
TTCAGAC95750.026.44316911
AGTTCAG108900.026.4252879
ACGTGTG64450.026.14127316
CGTGTGC62650.025.64732417
TCCGATC69050.025.51659227
AGACGTG78400.025.49788914
GCACACG23450.025.37442818
GAGTTCA120800.025.0085338
CTTCCGA81350.024.83262425
CCGATCT65250.024.61454228
GTTCAGA110100.024.17439710
CTGGAGT93350.024.0222025
Produced by FastQC (version 0.11.2)