FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n02 rad27-frb pnk.34200000008869.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n02 rad27-frb pnk.34200000008869.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17481283
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTA23150.014.436531
GGCGGTA32750.014.3913191
GGGGGAT40500.014.3880891
CGGGGAT25450.013.9175211
GCGGGTG20350.013.4751861
GGGGGAC30350.013.3646511
GGGGACT28750.013.353142
CGGGGAC17200.013.2858541
GCGGGTT22850.013.125961
CGGGGAG17500.013.0580991
GGCGGTT28650.012.8615421
GGGGAAC41100.012.7250122
GGCGGTC15000.012.3779881
GCGGGTC12800.012.2738471
GGGGTCA28800.012.2674242
AGGGGAC25700.012.1149421
GGGGGCG25850.011.9341411
GGGCGAC19650.011.9200831
TGGGGAC34700.011.8538921
TGGGGTA49550.011.7025461
Produced by FastQC (version 0.11.2)