FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n02 dna2-frb rnh201-frb pnk.342000000088d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n02 dna2-frb rnh201-frb pnk.342000000088d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25555064
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAC25400.014.8193261
CGGGGAT35750.014.5172231
CGGGGAA56800.014.3583971
CGGGGAG26850.014.3376411
GGCGGTC20450.014.2231271
CGGGGTA28750.013.6876691
GGGGACA53100.013.6611492
TGGGGTT93850.012.8526981
TGGGGAC50000.012.8321891
GGGGGAC42550.012.5322861
GGGGAAC57050.012.422982
CAGGGAC40650.012.3463991
TCCGATC34550.012.16513127
TGGGGTA73850.012.0859851
GGGGTAA62900.011.9303832
GGGGACC36200.011.9004772
CGGGGTT39300.011.89979651
GAGGGAC65200.011.8524991
GGGGGAT54250.011.8269021
GGGGAGC39450.011.5541522
Produced by FastQC (version 0.11.2)