##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l02n02 dna2-frb rad27-frb pnk.342000000088fd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17461802 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.15070569463564 27.0 15.0 33.0 2.0 33.0 2 24.10427239983594 27.0 15.0 33.0 2.0 33.0 3 23.286717373155415 27.0 15.0 33.0 2.0 33.0 4 26.59563675043389 33.0 15.0 37.0 2.0 37.0 5 28.306238668838418 33.0 15.0 37.0 15.0 37.0 6 28.390570629537546 33.0 22.0 37.0 6.0 37.0 7 28.83206212050738 33.0 22.0 37.0 15.0 37.0 8 29.76315342482981 33.0 27.0 37.0 15.0 37.0 9 30.368479438720012 33.0 27.0 37.0 15.0 37.0 10 30.253470346302173 33.0 27.0 37.0 15.0 37.0 11 29.792807523530502 33.0 27.0 37.0 15.0 37.0 12 29.76603102016619 33.0 27.0 37.0 15.0 37.0 13 29.879846421348724 33.0 27.0 37.0 15.0 37.0 14 31.10125638808641 33.0 27.0 37.0 15.0 40.0 15 30.147708638547154 33.0 27.0 37.0 15.0 40.0 16 30.88217006469321 33.0 27.0 37.0 15.0 40.0 17 30.68387260375533 33.0 27.0 37.0 15.0 40.0 18 30.400696274072974 33.0 27.0 37.0 15.0 40.0 19 30.843145512702527 33.0 27.0 37.0 15.0 40.0 20 30.518680488989624 33.0 27.0 37.0 15.0 40.0 21 30.670555765092285 33.0 27.0 37.0 15.0 40.0 22 30.635280711578336 33.0 27.0 37.0 15.0 40.0 23 30.611843210683524 33.0 27.0 37.0 15.0 40.0 24 30.75296472838256 33.0 27.0 37.0 15.0 40.0 25 30.746933621169223 33.0 27.0 37.0 15.0 40.0 26 31.567044684162607 33.0 27.0 37.0 15.0 40.0 27 30.229760823081147 33.0 27.0 37.0 15.0 40.0 28 31.09809869565581 33.0 27.0 37.0 15.0 40.0 29 30.786285058094233 33.0 27.0 37.0 15.0 40.0 30 30.84800875648458 33.0 27.0 37.0 15.0 40.0 31 31.154009591908096 33.0 27.0 37.0 15.0 40.0 32 30.762530579604555 33.0 27.0 37.0 15.0 40.0 33 30.999926410802274 33.0 27.0 37.0 15.0 40.0 34 30.96563464641278 33.0 27.0 37.0 15.0 40.0 35 29.207590488083646 33.0 27.0 37.0 6.0 40.0 36 30.135400916812596 33.0 27.0 37.0 15.0 40.0 37 30.25152364000004 33.0 27.0 37.0 15.0 40.0 38 30.623320433939178 33.0 27.0 37.0 15.0 40.0 39 30.257434026568394 33.0 27.0 37.0 15.0 40.0 40 30.244382681695736 33.0 27.0 37.0 15.0 40.0 41 30.793363537165295 33.0 27.0 37.0 15.0 40.0 42 30.440104864320418 33.0 27.0 37.0 15.0 40.0 43 29.880263674963214 33.0 27.0 37.0 15.0 40.0 44 30.252311531192486 33.0 27.0 37.0 15.0 40.0 45 30.43838568321872 33.0 27.0 37.0 15.0 40.0 46 30.746508922733174 33.0 27.0 37.0 15.0 40.0 47 30.63047072690436 33.0 27.0 37.0 15.0 40.0 48 30.451963491511357 33.0 27.0 37.0 15.0 40.0 49 29.853177008879154 33.0 27.0 37.0 15.0 40.0 50 30.46706703008086 33.0 27.0 37.0 15.0 40.0 51 29.448971933137255 33.0 27.0 37.0 15.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 8.012980543739353 2201 2 7.202643005735215 2201 3 7.474935337359145 2201 4 8.225318265012788 2201 5 6.322819451297548 2201 6 6.3729068360912215 2201 7 5.813711725686481 2201 8 4.845037189632901 2201 9 3.941325778667533 2201 10 3.966270400627849 2201 11 4.358706428474083 2201 12 4.637705997624746 2201 13 4.532031713376231 2201 14 4.530765875642409 2201 15 5.31525954191304 2201 16 4.685895987734035 2201 17 4.926799113481437 2201 18 4.037837379104456 2201 19 4.285297987144652 2201 20 3.2128629182260724 2201 21 0.3421331433485406 2201 22 3.178044476953314 2201 23 3.7701389378745205 2201 24 3.9980248112598815 2201 25 -1.8446602316594074 2201 26 0.8746146067508178 2201 27 2.9170972318565944 2201 28 -2.332799709303359 2201 29 1.546517137898487 2201 30 2.684689070925611 2201 31 3.034711393506825 2201 32 3.354750367568929 2201 33 2.9815797497748697 2201 34 2.734822583501643 2201 35 5.279945322873193 2201 36 3.4545364964790863 2201 37 3.745583299600561 2201 38 3.1364665402395318 2201 39 3.65244046833719 2201 40 3.798660448849393 2201 41 2.7075878202259567 2201 42 2.7649051415330597 2201 43 3.89193984188854 2201 44 3.255335603152428 2201 45 3.1348564031125896 2201 46 2.5170396738224063 2201 47 2.643424442192508 2201 48 2.5740023125003155 2201 49 3.050901468047261 2201 50 2.3268365213136697 2201 51 2.315760258476484 1101 1 8.088311157685531 1101 2 6.969585498112412 1101 3 7.752374859086082 1101 4 8.43132579481599 1101 5 6.60744469518999 1101 6 6.22307568688127 1101 7 5.881440927148596 1101 8 4.948681899823914 1101 9 4.170114634350284 1101 10 4.005083563474926 1101 11 -0.36206927213557805 1101 12 3.2597039070439813 1101 13 3.701349707041377 1101 14 4.076310738737625 1101 15 5.340125953575839 1101 16 4.871557982647538 1101 17 5.081433838764745 1101 18 5.056003457721577 1101 19 4.509458251118801 1101 20 4.94742415229328 1101 21 4.629239060878955 1101 22 4.649019666154221 1101 23 4.5451161860979425 1101 24 3.564286154304476 1101 25 4.233169071015535 1101 26 2.9159794251229414 1101 27 -0.21761071564293033 1101 28 2.1831068605157995 1101 29 2.5527605626733845 1101 30 3.5161505548360488 1101 31 3.130137424341264 1101 32 3.69315717607979 1101 33 3.3532694809702797 1101 34 3.254854365584233 1101 35 5.615284044435743 1101 36 3.5157732920166254 1101 37 3.512289286125146 1101 38 3.3563420688874004 1101 39 3.7091214100410355 1101 40 3.648150517765256 1101 41 3.060233846829984 1101 42 3.2731312434466737 1101 43 3.705120990041838 1101 44 3.068653841004835 1101 45 2.888543050043321 1101 46 2.664148528888145 1101 47 2.607058820358656 1101 48 2.2856438308031137 1101 49 2.737887504323492 1101 50 2.238544840271256 1101 51 2.115086868982676 2202 1 8.129767006727693 2202 2 7.262199047409464 2202 3 7.817594827647234 2202 4 7.625630478739112 2202 5 5.849011196781333 2202 6 6.342359705431388 2202 7 5.934400825510494 2202 8 4.868165842179213 2202 9 4.209141702407024 2202 10 4.319946660733287 2202 11 4.608757680519044 2202 12 4.825748611980028 2202 13 4.710213576667993 2202 14 4.464986622493612 2202 15 5.281297322264965 2202 16 4.7675947219282335 2202 17 4.867448201893808 2202 18 4.913287266292901 2202 19 4.403675428591079 2202 20 4.997614451306127 2202 21 4.966593741236689 2202 22 4.808609346755123 2202 23 4.612568290120841 2202 24 4.36460235895634 2202 25 4.263780073159406 2202 26 3.3680064405107046 2202 27 4.6079413954850565 2202 28 3.8080313174075613 2202 29 4.212075750030657 2202 30 3.7469883087586275 2202 31 3.587059493719501 2202 32 3.6249783594176 2202 33 -2.4393119459377957 2202 34 1.3812599746413348 2202 35 4.614779724763263 2202 36 4.034347532351937 2202 37 -2.535833978433441 2202 38 1.1956401189226327 2202 39 -2.23560669411593 2202 40 1.4534868886181052 2202 41 2.3774420454249388 2202 42 3.082249008362414 2202 43 3.4033045872713252 2202 44 3.444001747482705 2202 45 3.5576092885190924 2202 46 2.988377160899798 2202 47 2.867117759347156 2202 48 2.903731990231865 2202 49 3.3192607656212267 2202 50 2.798546461617196 2202 51 2.173165355371303 1102 1 7.525486887618861 1102 2 6.806652924642162 1102 3 7.019425635198356 1102 4 8.005343496335072 1102 5 6.043791792276416 1102 6 4.401669388949546 1102 7 5.168908997590805 1102 8 4.650813012463548 1102 9 3.414229006020534 1102 10 3.899007562166524 1102 11 4.56706391551408 1102 12 4.340325544102061 1102 13 4.457162820219441 1102 14 4.154380247363271 1102 15 5.091070649775769 1102 16 4.397941548505585 1102 17 4.72281605485631 1102 18 4.611176707507884 1102 19 4.5913496947404155 1102 20 -1.6709292945621534 1102 21 2.293991934901502 1102 22 3.4231529501993343 1102 23 3.8084239932696633 1102 24 4.13304979259393 1102 25 4.166078439177124 1102 26 3.20937988834568 1102 27 4.39324348661701 1102 28 3.620396995064077 1102 29 3.9919359347241965 1102 30 3.7620870445385997 1102 31 3.3513532319633583 1102 32 3.227393056181139 1102 33 2.881361809322751 1102 34 3.1146427492550934 1102 35 4.743729037529089 1102 36 -1.1030848345277455 1102 37 1.7515516763579768 1102 38 1.912985462147418 1102 39 -3.2237408324743875 1102 40 1.1784781440733276 1102 41 1.7963087406258857 1102 42 2.7849435153385578 1102 43 3.8656405482202665 1102 44 3.4938453654375827 1102 45 2.6721555460051825 1102 46 2.2877748855507 1102 47 2.602116369827815 1102 48 2.3444363550615215 1102 49 3.1358961535958194 1102 50 2.4402552776080206 1102 51 2.32422460631393 2203 1 7.938029507582225 2203 2 7.051728352470803 2203 3 7.7864137007921315 2203 4 7.6701526752841005 2203 5 5.265822801455215 2203 6 5.906512457607523 2203 7 5.445905154320883 2203 8 4.203455714559038 2203 9 3.291909853459842 2203 10 3.062907234613043 2203 11 3.9544112744877644 2203 12 4.205943135930351 2203 13 4.207187776939751 2203 14 3.7826527560729133 2203 15 5.005983670655059 2203 16 4.486241743217626 2203 17 4.663508198574611 2203 18 4.164333738572157 2203 19 4.114110707211324 2203 20 -2.1331415128517683 2203 21 1.7567903994960226 2203 22 3.3620510257423426 2203 23 2.8681149066310923 2203 24 3.43964012497419 2203 25 3.894114733000709 2203 26 3.078439593987497 2203 27 4.556696495363163 2203 28 3.441658018513948 2203 29 3.7345543126776874 2203 30 3.933058482676838 2203 31 3.0952651612090776 2203 32 -0.8197972886128788 2203 33 2.0556916136142753 2203 34 2.7284370783887546 2203 35 3.548734539295669 2203 36 3.5106871181392307 2203 37 1.60014789552147 2203 38 2.759449695829133 2203 39 3.3682782552192805 2203 40 -2.813393047129491 2203 41 0.6553354340827973 2203 42 0.6789517161851819 2203 43 3.074739877996418 2203 44 2.939233540614847 2203 45 2.552770405776922 2203 46 2.3218438757456603 2203 47 2.5952852090204637 2203 48 2.440927021479599 2203 49 3.1432229735921773 2203 50 1.8534548277639864 2203 51 2.0666562135408277 1103 1 7.60542087268923 1103 2 5.427833476967201 1103 3 7.565297731482179 1103 4 7.928580022761203 1103 5 6.266755133425008 1103 6 6.400582633983856 1103 7 5.252685816034827 1103 8 4.133546716970141 1103 9 3.417242246351215 1103 10 3.889759408341284 1103 11 4.689730327099273 1103 12 4.897211277165372 1103 13 4.588870574515251 1103 14 4.334527607954911 1103 15 4.186362939673405 1103 16 4.440776343582385 1103 17 4.666034418680539 1103 18 4.910595124338403 1103 19 4.5245157455416845 1103 20 4.203371628434773 1103 21 3.357297932262302 1103 22 3.805848030667594 1103 23 3.5090811737226204 1103 24 3.4751729730386103 1103 25 4.022518703593487 1103 26 3.3397187667935455 1103 27 4.432459129184551 1103 28 3.653203069569578 1103 29 3.9891949945099157 1103 30 3.7503229138779623 1103 31 3.502319957141111 1103 32 3.9566722093830187 1103 33 3.0170233361107 1103 34 -4.112937446989655 1103 35 3.523162997013774 1103 36 3.2602246808639777 1103 37 2.9479925371274334 1103 38 2.9558597308880223 1103 39 3.769912949864363 1103 40 3.577787405029685 1103 41 3.2555466084910982 1103 42 3.659604298332713 1103 43 4.367377399119338 1103 44 3.7041473488187826 1103 45 3.2021906365061206 1103 46 2.7901517531760796 1103 47 2.554964375772993 1103 48 2.389173639237274 1103 49 -3.8146805094991905 1103 50 -0.4686798037699482 1103 51 0.7339342941376295 2204 1 7.278811556843944 2204 2 6.426010975387332 2204 3 5.7110351528401395 2204 4 5.971652730355089 2204 5 4.360699618928653 2204 6 4.591365769774651 2204 7 4.377245664631843 2204 8 3.048446490763137 2204 9 3.3458025756820646 2204 10 3.102808350395982 2204 11 3.6262774541110225 2204 12 3.7937778299806446 2204 13 3.541253514352338 2204 14 3.176168241549604 2204 15 4.206133767561287 2204 16 3.8017741214226604 2204 17 3.2613897215175243 2204 18 4.13333401064472 2204 19 3.1661862472915345 2204 20 3.7355505862199436 2204 21 1.226551277237192 2204 22 1.7928310957467204 2204 23 -4.278366352155018 2204 24 0.8173928252523766 2204 25 1.1190374285874718 2204 26 1.1729637941269786 2204 27 3.4941405659619775 2204 28 2.2745463543707984 2204 29 1.7800777485466526 2204 30 1.7296817142487733 2204 31 2.111916002168279 2204 32 1.9245744655802959 2204 33 1.9266435755067413 2204 34 2.530212368523113 2204 35 4.688189742130856 2204 36 2.905598469387826 2204 37 -4.33540380969864 2204 38 0.2491108965086113 2204 39 0.6874155336988856 2204 40 1.9262737202707285 2204 41 1.6030463492287943 2204 42 2.330126608229076 2204 43 3.354990806346134 2204 44 2.37740247988609 2204 45 2.106916829402241 2204 46 1.649085388177351 2204 47 1.8076293325147041 2204 48 1.726381921321277 2204 49 2.1259319448416925 2204 50 1.454247661615561 2204 51 1.425462439426262 2205 1 -20.22926150435995 2205 2 -8.904938113816577 2205 3 -1.5149476796458714 2205 4 2.2632083697247403 2205 5 2.9246292221980603 2205 6 3.384051939135432 2205 7 2.362564831089241 2205 8 2.3226822610348243 2205 9 1.9680100797665219 2205 10 2.904135726171603 2205 11 2.4562605782038602 2205 12 2.784397241907417 2205 13 2.6460192159562297 2205 14 2.136851121800049 2205 15 1.7492453996842237 2205 16 0.5474531663535274 2205 17 0.9197719239753788 2205 18 1.282264441890188 2205 19 1.6225428002684694 2205 20 1.0429523658552569 2205 21 0.19765953644946777 2205 22 -0.38486070495702407 2205 23 1.5595725800249234 2205 24 1.9340087517993396 2205 25 1.1530924352491603 2205 26 1.25799847490336 2205 27 1.5032114039426432 2205 28 0.3789357733783163 2205 29 0.3020434387180373 2205 30 1.3371162131846432 2205 31 0.6489963171605915 2205 32 -0.05917169922976129 2205 33 0.2652346970031694 2205 34 0.6387658900569981 2205 35 3.823147963054584 2205 36 2.6579325613825944 2205 37 2.6414274933607516 2205 38 -0.2994458188117264 2205 39 1.8837473347634734 2205 40 1.187634338566948 2205 41 1.135116744057644 2205 42 1.8264547410427419 2205 43 1.6370362870089394 2205 44 1.8730969747447759 2205 45 1.4621877428040086 2205 46 0.5346557478005032 2205 47 1.0454489619581402 2205 48 0.9607295033182446 2205 49 1.7346638100928615 2205 50 1.0271883503497676 2205 51 1.2002582146134806 2206 1 4.444565958684606 2206 2 -1.7828860789188923 2206 3 1.7438389911032885 2206 4 3.013895701349373 2206 5 1.4585011710815863 2206 6 3.2137784261202462 2206 7 1.5774128092482798 2206 8 0.28318336369972386 2206 9 -0.3486768421719688 2206 10 -0.8092324340169803 2206 11 0.8377980369173486 2206 12 0.4754058217202619 2206 13 0.09596136711536118 2206 14 -1.1752236712268207 2206 15 0.31098938043571067 2206 16 1.0636676650630186 2206 17 0.6484586191225006 2206 18 0.6472561047437999 2206 19 0.4146495796100602 2206 20 0.9576941811288968 2206 21 1.7893864369841168 2206 22 2.7046303678839188 2206 23 2.2648723339218577 2206 24 2.0834890773885135 2206 25 0.6968412645683202 2206 26 -1.720042387795118 2206 27 0.2580126635064133 2206 28 0.20896502626459323 2206 29 0.7376459693517496 2206 30 0.19320061720716453 2206 31 0.809116448858056 2206 32 0.16264122106661105 2206 33 -0.2196974176192512 2206 34 -0.8849365553715636 2206 35 1.8857408543266736 2206 36 1.4261207683971087 2206 37 0.4548275748521782 2206 38 -0.8172970425726547 2206 39 -0.5711467349902186 2206 40 0.44821438709357153 2206 41 0.36949279265202506 2206 42 1.2449640649899614 2206 43 2.8571220982135195 2206 44 2.0325837981043335 2206 45 1.8375351700076052 2206 46 1.4685536058152415 2206 47 1.2156575215567607 2206 48 1.4292380182357824 2206 49 2.0933255559559782 2206 50 1.2162529203208514 2206 51 1.103957174523238 2207 1 3.2980890067272135 2207 2 -0.5627504964296577 2207 3 -2.1440337273674466 2207 4 -23.504001944451915 2207 5 -12.897648424001831 2207 6 -7.586491248968269 2207 7 -3.4481817713543066 2207 8 -2.8136123582734065 2207 9 -2.163173505341483 2207 10 -1.8392816137781658 2207 11 0.3425948815730351 2207 12 -0.34563707780209896 2207 13 0.6486182302478731 2207 14 -0.2352925619327806 2207 15 1.1593650030743952 2207 16 1.0341832509281943 2207 17 -1.5398580948481637 2207 18 1.0937500302183807 2207 19 -1.1987880693828217 2207 20 0.6117843347982941 2207 21 -0.8853957094565779 2207 22 0.5018629618248767 2207 23 -0.7202472947732339 2207 24 -1.429762396405426 2207 25 -0.08747247424385662 2207 26 -0.34262854280487076 2207 27 -27.65796095278266 2207 28 -16.469005361401134 2207 29 -7.154239603806303 2207 30 -3.5321504200429388 2207 31 -3.908859140992014 2207 32 -2.518708392352316 2207 33 -2.0431301613551476 2207 34 -4.605775625820371 2207 35 -26.332472248887864 2207 36 -16.26490778207129 2207 37 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-16.27816721893761 1213 26 -10.351993698622124 1213 27 -5.900440860934211 1213 28 -4.743663519615648 1213 29 -28.203094964506477 1213 30 -16.34668910005929 1213 31 -28.53251610843266 1213 32 -16.180957070133644 1213 33 -9.649826055016895 1213 34 -7.710288069742848 1213 35 -26.332472248887864 1213 36 -15.50782410421441 1213 37 -10.013915676768413 1213 38 -7.642502718764721 1213 39 -27.71174688620121 1213 40 -27.695957472019497 1213 41 -15.94620434081345 1213 42 -13.840050211275614 1213 43 -27.25800682219214 1213 44 -27.71142642167302 1213 45 -27.908951781526664 1213 46 -17.03980218732005 1213 47 -15.479345308074477 1213 48 -9.749835837339493 1213 49 -8.019910301193303 1213 50 -5.461144125446559 1213 51 -5.225597335231292 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 214.0 5 2751.0 6 10230.0 7 26110.0 8 53361.0 9 89717.0 10 118591.0 11 113915.0 12 150072.0 13 187988.0 14 221091.0 15 249103.0 16 266696.0 17 271993.0 18 287625.0 19 295543.0 20 315678.0 21 349990.0 22 386060.0 23 426289.0 24 472319.0 25 515067.0 26 537682.0 27 558889.0 28 639814.0 29 730068.0 30 732811.0 31 641964.0 32 738068.0 33 933291.0 34 1151514.0 35 1395475.0 36 1625551.0 37 1716167.0 38 1231498.0 39 18607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.11447553941311 17.312788943879063 24.320350504218478 16.252385012489352 2 40.161838813678955 26.16245715906798 27.06291227936725 6.612791747885817 3 38.136689367056476 22.379539848014083 31.5427619052875 7.941008879641945 4 45.80954448057731 19.788588070039218 25.421533315770557 8.980334133612915 5 45.598330999858874 22.588337902849123 22.491070105210078 9.322260992081933 6 15.578595478273638 32.88949036212865 39.89674636630436 11.63516779329335 7 22.187651375040737 29.100415882848658 31.76944379001194 16.942488952098664 8 22.30058247201974 30.109132424307127 30.932322771910297 16.65796233176283 9 22.0572228417733 29.670960243369716 30.98505767744489 17.286759237412095 10 24.08603567437541 28.59354226639776 29.738873826752155 17.581548232474677 11 23.384118246797975 29.346075807345244 29.764719971105887 17.505085974750894 12 22.956532902802923 29.526386824342687 29.715552121171328 17.801528151683062 13 23.4827192706822 29.37477060900554 29.199466976065075 17.943043144247188 14 23.085219371310327 30.153994130014638 28.96997832030772 17.790808178367314 15 21.858567373946183 29.71610687921084 29.818594809287074 18.606730937555902 16 23.159544368885644 29.60434593082261 29.13096031489847 18.105149385393275 17 22.909961371882016 30.218315918325228 28.863949256180486 18.00777345361227 18 22.062352878599913 30.20078985776192 29.613629561916007 18.123227701722165 19 22.826077815772074 29.999878268226144 29.03731514045112 18.13672877555066 20 22.202647051699067 30.265259010372926 29.542256146159325 17.989837791768686 21 21.630267660965483 30.36185466278792 29.660884463590087 18.346993212656514 22 22.77999461191716 29.928931559938466 29.035799782194783 18.255274045949587 23 22.421581020507904 30.07071020817583 29.01481285550399 18.492895915812277 24 22.043306523905684 30.038441211603402 29.45801613292688 18.460236131564038 25 22.45326724516319 29.903771828202576 29.28181493224989 18.361145994384355 26 22.387506169180018 30.244089355726288 29.03896172915029 18.329442745943403 27 21.27584115991099 30.162594191116405 30.041286114533953 18.52027853443865 28 22.601865489025702 29.75722093286821 29.226548325310297 18.41436525279579 29 22.35492138334402 30.209512529184284 29.103595776800866 18.33197031067083 30 21.713410399711744 30.063961329822874 29.609188591040407 18.613439679424975 31 22.43169895700595 30.076490318567632 29.047860728021867 18.443949996404545 32 22.12578633064331 30.23499521985188 29.29048788893609 18.348730560568722 33 21.625040868325275 30.24458801435175 29.443004742930572 18.6873663743924 34 22.491295660735762 29.99644994331306 29.151193987891094 18.36106040806009 35 22.232503479232303 30.39742137935989 29.097588369082505 18.2724867723253 36 21.63486334342813 30.391078767242924 29.35009227569984 18.62396561362911 37 22.17485702151415 30.05948784649941 29.456735013129865 18.308920118856577 38 22.30144918140976 30.349822689988983 28.827618979933845 18.521109148667414 39 21.561685380942013 30.224776653561904 29.59777250810624 18.615765457389845 40 22.318936333987693 29.980864326682116 29.203457713956592 18.4967416253736 41 22.446154182712643 30.30113959601649 28.904387989280835 18.34831823199003 42 21.682778830997055 30.122733791052713 29.630866165508795 18.56362121244144 43 22.13504851583928 29.95727036178058 29.567790212098384 18.339890910281753 44 22.229462171602442 30.24381963430241 29.152138005910533 18.374580188184613 45 21.525547745732574 30.262165379234453 29.71715917254143 18.495127702491548 46 22.394288195855857 30.11202383068474 29.074093339451302 18.419594634008103 47 22.138431907814304 30.255445101988666 29.10283669550514 18.503286294691893 48 21.549954149265954 30.13897457158445 29.62118843333301 18.689882845816587 49 21.90406927481234 29.69568761535797 29.624558458665295 18.775684651164394 50 22.38810987333358 30.314230820239136 28.74735187448327 18.550307431944006 51 21.908116449255964 30.224475163704344 29.282728516785223 18.58467987025447 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 244.0 1 422.0 2 600.0 3 963.5 4 1327.0 5 2074.0 6 2821.0 7 4390.5 8 5960.0 9 8996.0 10 12032.0 11 16939.5 12 21847.0 13 28726.0 14 35605.0 15 44782.0 16 53959.0 17 65183.0 18 76407.0 19 92015.5 20 107624.0 21 131735.0 22 155846.0 23 191907.0 24 227968.0 25 281955.5 26 411162.0 27 486381.0 28 585159.5 29 683938.0 30 798655.0 31 913372.0 32 1034921.0 33 1156470.0 34 1269381.0 35 1382292.0 36 1469391.5 37 1556491.0 38 1600893.0 39 1645295.0 40 1635762.0 41 1626229.0 42 1572909.5 43 1519590.0 44 1428219.5 45 1336849.0 46 1227477.5 47 1118106.0 48 1002982.5 49 887859.0 50 776166.0 51 664473.0 52 568762.5 53 473052.0 54 397573.5 55 322095.0 56 267009.5 57 211924.0 58 173227.0 59 134530.0 60 111851.5 61 89173.0 62 75295.5 63 61418.0 64 52462.5 65 43507.0 66 37694.5 67 31882.0 68 27740.5 69 23599.0 70 20765.0 71 17931.0 72 15610.5 73 13290.0 74 11404.0 75 7912.5 76 6307.0 77 5116.0 78 3925.0 79 3010.5 80 2096.0 81 1583.0 82 1070.0 83 798.5 84 527.0 85 370.0 86 213.0 87 156.0 88 99.0 89 72.5 90 46.0 91 33.5 92 21.0 93 21.0 94 21.0 95 15.5 96 10.0 97 9.5 98 9.0 99 10.0 100 11.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 20.776876292607145 2 10.108195019047862 3 16.984054681183533 4 14.09005783022852 5 3.0186575245785057 6 6.708534434189553 7 4.616648384857417 8 2.616368001423908 9 0.9975774550644887 10 4.214358861702819 11 4.214358861702819 12 6.2354389312168355 13 2.8230648818489636 14 0.9975774550644887 15 4.214513484919827 16 4.214358861702819 17 4.214358861702819 18 3.4232034013442596 19 2.618303655029418 20 2.5959176492781215 21 2.595568315343399 22 1.6215394035506758 23 1.6187905463594192 24 2.595568315343399 25 1.598345920999448 26 0.0 27 3.3734147254676237 28 0.0 29 0.9978580675694295 30 1.6188019999310495 31 1.0084010802550618 32 0.0 33 0.0013457946665527418 34 6.242196538478674E-4 35 6.174385667641862 36 0.0 37 4.810500084699162E-4 38 7.444821559653465E-5 39 0.997588908636119 40 0.9975774550644887 41 0.0 42 0.015078627051205825 43 2.595568315343399 44 1.151610813133719 45 1.2272043858932773 46 0.2534560866054947 47 0.29821091775064223 48 0.31055786796803675 49 0.30942969116245844 50 0.3374565809416462 51 0.31738992344547257 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1.7461802E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.61152699625124 #Duplication Level Percentage of deduplicated Percentage of total 1 88.16401338599685 72.83363771935134 2 8.363212111873564 13.817954463108364 3 2.1328816381578837 5.286018270914658 4 0.68086312113972 2.2498856845114346 5 0.26030073330135345 1.0751920528134375 6 0.13118937934487748 0.6502652972022473 7 0.06056283649405339 0.35022318813986286 8 0.039753479586836137 0.26272765216662686 9 0.030295313614110475 0.22524679066427872 >10 0.1193007290196605 1.8078314663915125 >50 0.011811749355707613 0.6857603795521101 >100 0.005737716062765973 0.6861921487592996 >500 5.2007945272225686E-5 0.02928035009877177 >1k 2.579810727395497E-5 0.03978453632601443 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.05642029385054303 0.0 0.0 2 0.05701015278950019 5.72678581511805E-6 0.0 3 0.057577104585196874 5.72678581511805E-6 5.72678581511805E-6 4 0.061631668942300454 5.72678581511805E-6 5.72678581511805E-6 5 0.06300609753792878 5.72678581511805E-6 5.72678581511805E-6 6 0.06908794407358416 5.72678581511805E-6 5.72678581511805E-6 7 0.182936446078131 5.72678581511805E-6 5.72678581511805E-6 8 0.18653859435584025 5.72678581511805E-6 1.14535716302361E-5 9 0.19286096589573057 5.72678581511805E-6 1.14535716302361E-5 10 0.2261794057680874 5.72678581511805E-6 1.14535716302361E-5 11 0.30416677499836503 5.72678581511805E-6 1.14535716302361E-5 12 0.3447467792842915 5.72678581511805E-6 1.14535716302361E-5 13 0.38997693365209385 5.72678581511805E-6 1.14535716302361E-5 14 0.4052731785642742 5.72678581511805E-6 1.14535716302361E-5 15 0.4239997681797102 5.72678581511805E-6 1.14535716302361E-5 16 0.44219376671434024 5.72678581511805E-6 1.14535716302361E-5 17 0.4597749991667527 5.72678581511805E-6 1.14535716302361E-5 18 0.48048305667421953 5.72678581511805E-6 1.14535716302361E-5 19 0.5020558588397692 5.72678581511805E-6 1.14535716302361E-5 20 0.5190873198539303 1.14535716302361E-5 1.14535716302361E-5 21 0.5398068309330274 1.718035744535415E-5 1.718035744535415E-5 22 0.557101724094684 2.863392907559025E-5 2.29071432604722E-5 23 0.5806559941522644 3.43607148907083E-5 2.29071432604722E-5 24 0.6018680088114617 4.008750070582635E-5 2.863392907559025E-5 25 0.6276786324801987 4.008750070582635E-5 2.863392907559025E-5 26 0.6502879828782848 4.008750070582635E-5 2.863392907559025E-5 27 0.6748730743825866 4.008750070582635E-5 2.863392907559025E-5 28 0.6988053123039649 4.008750070582635E-5 2.863392907559025E-5 29 0.7233789502366366 4.008750070582635E-5 2.863392907559025E-5 30 0.7511137739392533 4.008750070582635E-5 2.863392907559025E-5 31 0.780354742311246 4.008750070582635E-5 3.43607148907083E-5 32 0.8130146018148643 4.008750070582635E-5 3.43607148907083E-5 33 0.8475184863509505 4.008750070582635E-5 3.43607148907083E-5 34 0.8834655209124465 4.008750070582635E-5 3.43607148907083E-5 35 0.9299784753028353 4.008750070582635E-5 3.43607148907083E-5 36 0.9715205796057016 4.008750070582635E-5 4.008750070582635E-5 37 1.0152216821608675 4.58142865209444E-5 4.008750070582635E-5 38 1.0594038347245032 4.58142865209444E-5 4.008750070582635E-5 39 1.1076920927175786 4.58142865209444E-5 4.008750070582635E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCGCC 2135 0.0 21.663422 44 GTCGCCG 2450 0.0 19.398775 45 CGGGGAC 1540 0.0 17.641985 1 GCGGGTT 2410 0.0 16.019957 1 GGGGGAC 3150 0.0 14.980212 1 GGGGGAT 4285 0.0 14.816538 1 AGGGGAC 3035 0.0 14.793997 1 GCGGGTA 2200 0.0 14.1693 1 CGGGGAT 2470 0.0 14.125618 1 GGGGACC 2400 0.0 14.107362 2 CGGGGAG 1860 0.0 13.991782 1 TGGGGTT 7025 0.0 13.8006 1 GGCGGTC 1625 0.0 13.727322 1 GGGGTTA 4515 0.0 13.707061 2 GGGGTTC 4035 0.0 13.686953 2 GCGGGTC 1340 0.0 13.659026 1 CGGGGTT 2330 0.0 13.501478 1 GGGGTCA 3125 0.0 13.409857 2 GGGGACT 2920 0.0 13.400885 2 AGGGGTT 4810 0.0 13.377718 1 >>END_MODULE