FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n02 dna2-frb rad27-frb pnk.342000000088fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n02 dna2-frb rad27-frb pnk.342000000088fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17461802
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC21350.021.66342244
GTCGCCG24500.019.39877545
CGGGGAC15400.017.6419851
GCGGGTT24100.016.0199571
GGGGGAC31500.014.9802121
GGGGGAT42850.014.8165381
AGGGGAC30350.014.7939971
GCGGGTA22000.014.16931
CGGGGAT24700.014.1256181
GGGGACC24000.014.1073622
CGGGGAG18600.013.9917821
TGGGGTT70250.013.80061
GGCGGTC16250.013.7273221
GGGGTTA45150.013.7070612
GGGGTTC40350.013.6869532
GCGGGTC13400.013.6590261
CGGGGTT23300.013.5014781
GGGGTCA31250.013.4098572
GGGGACT29200.013.4008852
AGGGGTT48100.013.3777181
Produced by FastQC (version 0.11.2)