FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n02 dna2-frb- pnk.34200000008883.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n02 dna2-frb- pnk.34200000008883.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17481999
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC43200.032.70719544
GTCGCCG45000.031.49557345
GCACACG38300.028.79969618
GTGACTG59650.027.3593181
GACTGGA62250.026.9714243
TGGTCGC51200.026.02737643
TGACTGG63350.025.669392
GTGGTCG56600.024.07614342
AGCACAC52600.023.00358817
AGAGCAC66300.022.7875515
GAGCACA68150.022.07086616
CTCGGTG69100.021.74397738
ACACTCT43550.020.60731
CGGGGAC16300.020.3399541
CACACGT27650.019.6090119
ACACGAG29850.019.55186720
GGTGGTC72750.019.23055641
TCTCGGT79600.019.22572737
CACACGA31850.019.15110619
ACTGGAG77250.018.9295334
Produced by FastQC (version 0.11.2)