FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n01 wt - biotin-dc.34100000008935.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n01 wt - biotin-dc.34100000008935.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13213721
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT35450.014.2323143
CGTATGC41650.012.5842445
ATCTCGT39800.012.45035141
AATCTCG42250.011.62175740
TCTCGTA48250.011.29696242
TGCCCCG15650.011.2105495
TCGTATG47450.011.06020844
CCCCGGG16050.010.9304567
GTGCCCC17750.010.6445564
CCCGGGT17550.09.9962288
CGTGTGC31500.09.5889361
CCTTGTA95200.09.39284644
TGTAATC60150.08.98709343
GTAATCT59800.08.92669744
GACGTGT23400.08.8623341
GCCCCGG21550.08.3495196
CTTGTAA111650.08.15985445
ACCTTGT112650.08.11780343
CCGGGTA23850.08.1101479
AGTCACC116200.07.81049139
Produced by FastQC (version 0.11.2)