FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n01 rnh201-frbrad27-frb pnk.341000000088a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n01 rnh201-frbrad27-frb pnk.341000000088a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11031084
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC143850.13040422863247167TruSeq Adapter, Index 7 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC116670.10576476436948536TruSeq Adapter, Index 14 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC38050.023.5303945
CATCTCG36250.022.6183840
TCGTATG40850.022.22199444
CTCGTAT40950.020.73755343
CAGATCA200250.019.9625345
ATCTCGT44700.018.74577941
TCTCGTA47800.017.57725342
GACGTGT40550.014.8960241
GTTGGGA31000.013.49972229
TGTTGGG33500.012.82838328
AGATCAT118600.012.78426945
GGGATTC33100.012.71064132
GTCACAG60650.011.9824430
CGTGTGC68950.011.7350941
TCATCTC73100.011.492022539
ACGTGTG53200.011.4577482
CACAGAT119300.011.44727432
TGGGATT39500.011.22067631
AGTCACA59850.010.9020329
GTGAATG33950.010.79865711
Produced by FastQC (version 0.11.2)