FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n01 rnh201-frb pnk.34100000008850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n01 rnh201-frb pnk.34100000008850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11072309
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGT49900.012.14209844
CGATGTA49700.012.03975745
GTTGGGA27900.011.29088829
GTCACCG61800.010.78810840
GGGATTC28250.010.75163532
ACCGATG60550.010.49003643
TGTTGGG29750.010.36233128
CACCGAT64950.010.05682542
TCACCGA64050.010.02230841
CCCCGGG10800.09.7879127
AGTCACC67950.09.71076539
GACGTGT22550.09.696281
CGTGTGC31850.09.2713261
TATCTCG21700.08.9264940
TGCCCCG11950.08.84649855
GTGAATG31550.08.83971511
GTTGAGA33650.08.7566817
CAGTCAC76450.08.631124538
TGGGATT38100.08.62173931
CCCGGGT12000.08.6216938