FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n01 rnh201-frb rad27-frb dna2-frb pnk.34100000008928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n01 rnh201-frb rad27-frb dna2-frb pnk.34100000008928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15721949
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC274330.17448854464545077TruSeq Adapter, Index 11 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC242430.1541984393919609TruSeq Adapter, Index 14 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGGGA62250.025.08539229
TGTTGGG63450.024.71798128
GGGATTC67100.023.53846232
CGTATGC70400.023.167245
CATCTCG63900.023.08631140
CTCGTAT68650.022.50603943
TGGGATT75900.021.01646431
GTGAATG62200.021.00934411
TCGTATG78950.020.99622344
TTGGGAT78350.020.59116730
GTTGAGA68600.020.16584417
ATCTCGT75050.019.32632641
TGAGATA73750.019.03215619
ATGGGTG44150.018.6965777
GGATTCC85800.018.560633
GAGATAA80000.017.68540420
TCTCGTA84250.017.45656442
GTTGATA94750.016.16386643
TGAATGT83000.016.1240412
GGGTGAA53450.015.7801199
Produced by FastQC (version 0.11.2)