FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n01 rad27-frb pnk.3410000000886c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n01 rad27-frb pnk.3410000000886c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17481283
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGGG42700.09.43262528
GTTGGGA40700.09.343129
GGGATTC43350.08.87492532
TGGGATT55250.07.12622931
GACGTGT27800.06.9745011
GTGAATG47700.06.837217311
GTTGAGA50550.06.67472517
CGTGTGC28850.06.6270693
TTGGGAT61050.06.597297730
TGAGATA54850.06.52063619
GAGATAA59550.06.23236720
AGACGTG27450.06.06370075
GGATTCC65750.06.05507733
ACGTGTG30350.06.00341462
CAGACGT27500.05.80729634
ATGGGTG34500.05.54152877
TCAGACG28850.05.37962063
GAATGTT72800.05.376178313
GATTCCA82100.05.342273734
TCCGATC38900.05.261960513
Produced by FastQC (version 0.11.2)