FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n01 dna2-frb rnh201-frb pnk.341000000088d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n01 dna2-frb rnh201-frb pnk.341000000088d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25555064
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGGG16500.08.8603127
CCCGGGT19500.07.6125298
TGCCCCG22350.06.44083935
GTGCCCC24800.06.3487234
CCGGGTA29300.06.1410419
GAACTCC119800.05.89578722
GACGTGT46050.05.82517861
GCCCCGG30850.05.6866836
CCCTACG25100.05.653311740
ACAGATC94350.05.43540733
TCAGACG48100.05.37765263
GTCACAG51050.05.33336530
GTCACAC64450.05.311984540
ACGTCTG131850.05.288398316
ACGTGTG48950.05.28450972
CGTGTGC47250.05.28398043
CGTCTGA136250.05.23331917
CGTATGC42700.05.057373545
GTCTGAA148350.05.034032318