FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n01 dna2-frb rad27-frb pnk.341000000088f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n01 dna2-frb rad27-frb pnk.341000000088f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17461802
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG40200.013.11103140
CGTATGC46000.012.91057445
CTCGTAT44800.012.4184143
TCGTATG53150.011.65403944
ATCTCGT53100.010.05312241
TCTCGTA58200.09.67529242
GGGATTC38350.09.2694932
GTTGGGA37700.09.19148729
CGATCAG87550.08.92730544
AGATCTC66150.08.37505239
TGTTGGG41900.08.27045328
GATCAGA99450.07.82655745
TGGGATT50500.07.39568431
CACGATC111100.07.27825942
GTCACGA112650.06.998161340
AGTCACG112900.06.861938539
AGACGTG29550.06.62213135
CAGACGT31000.06.60261064
CAGATCT87750.06.570166638
TCACGAT122300.06.556477541
Produced by FastQC (version 0.11.2)