FastQCFastQC Report
Sun 19 Apr 2015
HCNNTADXX l02n01 dna2-frb- pnk.34100000008886.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHCNNTADXX l02n01 dna2-frb- pnk.34100000008886.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17481999
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC323790.18521337291004306TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC76000.024.30043245
CTCGTAT76850.023.56397443
TATCTCG76700.023.1401440
TCGTATG82450.022.7010944
TCTCGTA88900.020.06595442
ATCTCGT88500.019.72393441
GCCAATA239800.014.23052245
ATATCTC133900.013.57323439
CGCCAAT262650.013.12907544
ACGTGTG66750.011.9195451
ACGCCAA295700.011.73782643
CACGCCA294100.011.73275842
CGTGTGC86250.011.524331
TCACGCC299550.011.49670241
GTCACGC307500.011.18482140
GACGTGT49700.010.9747651
AGTCACG326800.010.63345139
GTGTGCT99050.010.1287282
CAATATC184300.010.10629437
GTCACAG75800.09.9404086
Produced by FastQC (version 0.11.2)