##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename HCNNTADXX l01n02 wt-cdc9deg.34200000008876.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2454829 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 2.0 2.0 2.0 2.0 2.0 2.0 2 7.830585755667706 6.0 2.0 15.0 2.0 15.0 3 2.0 2.0 2.0 2.0 2.0 2.0 4 3.37459309793065 2.0 2.0 2.0 2.0 6.0 5 9.524900105058235 15.0 2.0 15.0 2.0 15.0 6 7.283041303487941 2.0 2.0 15.0 2.0 15.0 7 12.36520099770697 15.0 2.0 15.0 2.0 27.0 8 17.537997555023182 15.0 15.0 22.0 6.0 27.0 9 12.861402566125788 15.0 2.0 22.0 2.0 27.0 10 17.856094253408283 15.0 15.0 27.0 2.0 33.0 11 18.121424750970434 22.0 6.0 27.0 2.0 33.0 12 18.980645902423348 15.0 15.0 27.0 2.0 33.0 13 18.676097194550007 15.0 15.0 27.0 2.0 33.0 14 21.526956867464087 22.0 15.0 27.0 6.0 33.0 15 20.296159936191074 22.0 6.0 27.0 2.0 33.0 16 20.965653412111394 27.0 15.0 33.0 2.0 33.0 17 19.590442348530182 27.0 6.0 33.0 2.0 33.0 18 22.46415738122696 27.0 15.0 33.0 15.0 33.0 19 23.28234349520883 27.0 15.0 33.0 6.0 33.0 20 24.64841217046075 27.0 22.0 33.0 6.0 33.0 21 21.466139596688812 27.0 15.0 33.0 2.0 33.0 22 23.16641892368063 27.0 15.0 33.0 6.0 33.0 23 22.033883826531298 27.0 15.0 33.0 2.0 33.0 24 24.13261901338138 27.0 15.0 33.0 15.0 33.0 25 24.082789473319732 27.0 15.0 33.0 6.0 33.0 26 22.421719802071753 27.0 15.0 33.0 2.0 33.0 27 22.280907549975986 27.0 15.0 33.0 2.0 33.0 28 22.734135453019334 27.0 15.0 33.0 2.0 33.0 29 22.285943745979864 27.0 15.0 33.0 2.0 33.0 30 22.844675535444626 27.0 15.0 33.0 6.0 33.0 31 22.850713023188174 27.0 15.0 33.0 6.0 33.0 32 24.308255687056004 27.0 22.0 33.0 6.0 33.0 33 22.085556672175535 27.0 15.0 33.0 2.0 33.0 34 23.14447523636066 27.0 15.0 33.0 6.0 33.0 35 22.30456418756663 27.0 15.0 33.0 6.0 33.0 36 23.20959993547412 27.0 15.0 33.0 6.0 33.0 37 23.778414300955383 27.0 15.0 33.0 6.0 33.0 38 24.777479816313072 27.0 22.0 33.0 6.0 33.0 39 24.687937530475647 27.0 22.0 33.0 6.0 33.0 40 20.982518945311465 27.0 6.0 33.0 2.0 33.0 41 22.80231616947657 27.0 15.0 33.0 6.0 33.0 42 24.078565146492892 27.0 22.0 33.0 6.0 33.0 43 24.386549124195618 27.0 22.0 33.0 6.0 33.0 44 24.638159725178415 27.0 22.0 33.0 6.0 33.0 45 25.179676466263025 27.0 22.0 33.0 15.0 33.0 46 25.38578450881915 27.0 22.0 33.0 15.0 33.0 47 25.631193455837455 27.0 22.0 33.0 15.0 33.0 48 25.605306520331965 27.0 22.0 33.0 15.0 33.0 49 25.83299203325364 27.0 22.0 33.0 15.0 33.0 50 25.988771926680027 27.0 22.0 33.0 15.0 33.0 51 24.60809367984491 27.0 22.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 0.0 2201 2 -5.751737020333849 2201 3 0.0 2201 4 9.717268957345972 2201 5 3.7014344097727516 2201 6 11.87154920537741 2201 7 11.467470081538986 2201 8 5.87044115633055 2201 9 10.354275958226673 2201 10 5.0703023924338275 2201 11 8.40658509594175 2201 12 7.441486228285694 2201 13 8.193543634437109 2201 14 6.495614423527236 2201 15 3.2945838194654264 2201 16 4.055701264443066 2201 17 6.858570484091388 2201 18 2.623111652084148 2201 19 2.556894862133692 2201 20 1.582474564401224 2201 21 5.355782358500708 2201 22 4.37654803260574 2201 23 2.6891587114564395 2201 24 2.464923862587572 2201 25 -0.14071296099580266 2201 26 3.8698249898829005 2201 27 4.388179787045544 2201 28 2.958990328911863 2201 29 4.291246083032014 2201 30 2.274715693072171 2201 31 2.902125669788539 2201 32 1.430270519321077 2201 33 2.15562708503208 2201 34 -0.3993699035804781 2201 35 -3.572071150463298 2201 36 -2.3422782588624678 2201 37 -0.6920417680465505 2201 38 -0.44779265449564676 2201 39 0.33910040995845137 2201 40 3.06996591652565 2201 41 0.16642672071593978 2201 42 -1.834578637361414 2201 43 -2.348550673660938 2201 44 -1.5668894051214544 2201 45 -3.0549854102668235 2201 46 -2.051945701896855 2201 47 -0.787312623031557 2201 48 -0.6667468889943073 2201 49 -0.7004456969603439 2201 50 -1.1952270308855084 2201 51 -1.4010453217942 1101 1 0.0 1101 2 6.519562986235158 1101 3 0.0 1101 4 -1.388181279620853 1101 5 4.564966000350838 1101 6 8.119421302288636 1101 7 8.557079579991635 1101 8 3.8804826637921863 1101 9 -10.652997606807556 1101 10 -4.830574232353426 1101 11 0.04176658397032895 1101 12 3.295403631993956 1101 13 7.005044939486837 1101 14 1.9669838141561513 1101 15 2.64681513469521 1101 16 5.494691774475815 1101 17 6.192551726427332 1101 18 3.3871482268922932 1101 19 0.369624414178233 1101 20 0.8375402649395127 1101 21 0.04192905087056431 1101 22 1.7775529653457092 1101 23 3.8709062205867184 1101 24 1.1683089284974777 1101 25 0.5768702370052559 1101 26 0.7338696176922461 1101 27 -0.5914967797967705 1101 28 2.0896060649120685 1101 29 2.6491136429522406 1101 30 2.367056313005211 1101 31 1.6488083037439694 1101 32 0.4327139808012497 1101 33 2.2220119576249893 1101 34 2.7450212922096 1101 35 1.6848403019283325 1101 36 0.6802381880434005 1101 37 1.2450599361525327 1101 38 0.785283598535603 1101 39 0.7075207201152267 1101 40 0.9558115717637321 1101 41 -0.635319119699659 1101 42 1.2304240303681944 1101 43 0.399282217622126 1101 44 -1.549650510540463 1101 45 -2.5257923793610466 1101 46 -2.3460483300809685 1101 47 -1.2352403472247033 1101 48 -0.397575728144151 1101 49 -1.6963504881397604 1101 50 -1.172821753793258 1101 51 -1.4436054050355303 1102 1 0.0 1102 2 6.164956859882988 1102 3 0.0 1102 4 -1.388181279620853 1102 5 4.39462131735354 1102 6 6.642101000991937 1102 7 8.130562654659345 1102 8 6.959559168264892 1102 9 -10.652997606807556 1102 10 -3.294133756649858 1102 11 -15.952518379571679 1102 12 -6.497843766886543 1102 13 -2.6322328162996236 1102 14 -1.1029904340562169 1102 15 -1.5897623282600755 1102 16 -19.065184728184633 1102 17 -17.522189126229833 1102 18 -8.386295405843379 1102 19 -5.456305315427631 1102 20 -3.4628829711761284 1102 21 0.04191908216261098 1102 22 1.2081824106745707 1102 23 4.881378383687327 1102 24 2.557231674477471 1102 25 0.7934604748372607 1102 26 4.735204956906095 1102 27 4.143582609667639 1102 28 4.3554409906680185 1102 29 1.1920465319928262 1102 30 0.8859491297361721 1102 31 -0.8900873529639846 1102 32 0.07731411833466595 1102 33 2.5300493387824687 1102 34 0.09552306531174182 1102 35 0.781421492884359 1102 36 2.0242205827431157 1102 37 1.0217796462849016 1102 38 0.8501456950937651 1102 39 -1.5439890757781924 1102 40 2.2182067569777573 1102 41 0.45975208765863584 1102 42 -0.17901951438027552 1102 43 0.9255606070254103 1102 44 1.1341349382718988 1102 45 0.6432460017434352 1102 46 1.0493523470437864 1102 47 0.10547340745952027 1102 48 1.4016701192561527 1102 49 -0.016262024877207182 1102 50 0.4926170206141869 1102 51 0.6025991895001361 1103 1 0.0 1103 2 5.113717857269366 1103 3 0.0 1103 4 -1.388181279620853 1103 5 4.811445029172651 1103 6 -5.326614301731596 1103 7 1.251189081193246 1103 8 -0.389724990284698 1103 9 9.381498800450998 1103 10 7.218506067406118 1103 11 7.317820190639015 1103 12 7.095380106051103 1103 13 -16.726315501950463 1103 14 -7.736633687270073 1103 15 -18.44679322108223 1103 16 -7.218272050069908 1103 17 -0.347636696562315 1103 18 0.3081170094025687 1103 19 1.2587951926347358 1103 20 1.1303826537307806 1103 21 2.7435630116645093 1103 22 0.654452480324796 1103 23 1.9264261216831144 1103 24 1.9156507705634844 1103 25 0.4217865940841463 1103 26 2.256100057640616 1103 27 2.2491115352513837 1103 28 1.2415527047791706 1103 29 3.9475293017250834 1103 30 0.37352403122988065 1103 31 -0.5909723455698135 1103 32 0.011659335579771835 1103 33 2.2304060126756724 1103 34 0.36727009433777624 1103 35 1.4586174987171 1103 36 0.896273647579072 1103 37 1.5197543059019516 1103 38 -0.9681128507106784 1103 39 0.011176282343260624 1103 40 4.5971392283258155 1103 41 2.0756306952516965 1103 42 1.3206808323169739 1103 43 1.8401692730717905 1103 44 1.5169282537505993 1103 45 1.6197021187732261 1103 46 1.0903616279998687 1103 47 -0.11780379504813254 1103 48 -0.39917727271452463 1103 49 -0.10131063116123684 1103 50 0.16709653546664427 1103 51 0.6942569261054388 2103 1 0.0 2103 2 5.208710377947883 2103 3 0.0 2103 4 -1.388181279620853 2103 5 4.854707107238918 2103 6 -5.326614301731596 2103 7 -10.254291371857539 2103 8 -3.6996979667679977 2103 9 2.9083899762691168 2103 10 3.428994730229075 2103 11 5.837690730494781 2103 12 6.0156866973281495 2103 13 6.176164640698264 2103 14 4.048457749066245 2103 15 5.050629375773013 2103 16 5.625105432295186 2103 17 7.182333486835493 2103 18 2.5915549788096754 2103 19 0.8132755047085389 2103 20 2.069241738915526 2103 21 4.83678551805837 2103 22 1.3630807168358032 2103 23 3.2277425932138293 2103 24 1.8244214814809325 2103 25 1.1283153435669817 2103 26 5.2999955943875285 2103 27 4.937708230704324 2103 28 3.0295376586400558 2103 29 3.4208708307151205 2103 30 0.9053608853059103 2103 31 4.1639505367041 2103 32 -0.219094692475732 2103 33 3.8586431623354827 2103 34 1.3184721338587515 2103 35 -0.1237416137805809 2103 36 0.8820378990816131 2103 37 -2.161121138851211 2103 38 -0.012513001546771818 2103 39 -1.022917109606965 2103 40 1.4797006430895543 2103 41 1.05084740467046 2103 42 -0.5025821130114174 2103 43 1.2673126261314351 2103 44 0.6335013792971651 2103 45 1.0215255941513846 2103 46 0.8130344471539601 2103 47 1.1131339107551135 2103 48 0.8054566679451227 2103 49 0.9228694937138506 2103 50 1.2249919838487635 2103 51 0.9862399287548627 2102 1 0.0 2102 2 -5.751737020333849 2102 3 0.0 2102 4 -1.388181279620853 2102 5 -7.442391287962899 2102 6 -5.326614301731596 2102 7 1.3565727181894118 2102 8 -4.5457290602579565 2102 9 7.866886725147296 2102 10 3.5285701052964704 2102 11 5.539941432001346 2102 12 4.167137311724357 2102 13 8.124715249580895 2102 14 3.9841139455967784 2102 15 6.484956413568433 2102 16 3.650247005903239 2102 17 7.8657341348627945 2102 18 4.63290405647583 2102 19 2.207715543629874 2102 20 1.3801075300609824 2102 21 2.610604740097486 2102 22 -1.594812683904511 2102 23 1.0148357968646202 2102 24 -1.9364518416246739 2102 25 1.047954292944553 2102 26 2.7190266205543097 2102 27 2.495156879114525 2102 28 3.6442303876546234 2102 29 1.211688539895512 2102 30 -0.5153750558747703 2102 31 1.0527391478662445 2102 32 -0.3270279531041993 2102 33 2.60873616907719 2102 34 1.6088622342537562 2102 35 1.9238850032527886 2102 36 -0.7330315282422895 2102 37 2.1136020691106907 2102 38 -0.5593153418490431 2102 39 -0.43153728691646975 2102 40 2.071782265938939 2102 41 3.0642933993468304 2102 42 2.58315660846986 2102 43 3.0863416220686553 2102 44 0.6754081773550347 2102 45 1.6691405751669457 2102 46 1.464237596828312 2102 47 1.1992817839769963 2102 48 0.9166290066177183 2102 49 1.3861379368008073 2102 50 0.9387628671703467 2102 51 0.9791949757173022 2101 1 0.0 2101 2 -5.751737020333849 2101 3 0.0 2101 4 -1.388181279620853 2101 5 -7.442391287962899 2101 6 -5.326614301731596 2101 7 -10.254291371857539 2101 8 -4.374383573887062 2101 9 -10.652997606807556 2101 10 -15.689209192610932 2101 11 -15.952518379571679 2101 12 -4.722756973907227 2101 13 -4.2223205043822 2101 14 -2.327963190594396 2101 15 1.6537070220915346 2101 16 3.5483774200818843 2101 17 7.292825116804977 2101 18 2.8780270256748253 2101 19 3.5163848143539695 2101 20 1.5709764520208829 2101 21 3.7326307205873768 2101 22 1.4512943255183721 2101 23 2.321608574268968 2101 24 2.093019160214663 2101 25 1.487170778382655 2101 26 -20.21561471522505 2101 27 -20.099445278778575 2101 28 -20.561546795890663 2101 29 -20.085596992270776 2101 30 -8.688435759437857 2101 31 -8.902938708787335 2101 32 -3.7108851376090186 2101 33 -19.938665963941062 2101 34 -9.96246995812399 2101 35 -4.377872735836238 2101 36 -3.8397849525894188 2101 37 -1.619293355792145 2101 38 -0.7304350234694752 2101 39 0.08201319948258501 2101 40 4.497801581842889 2101 41 2.3006643899876913 2101 42 1.4366644331558334 2101 43 -2.128693178188641 2101 44 -0.30717024520933833 2101 45 0.7410444652025241 2101 46 -0.3104969627728593 2101 47 -0.4245619297768677 2101 48 -0.9137026733157683 2101 49 -0.2069985009369688 2101 50 -0.8040475171144976 2101 51 -0.29022907465121506 2104 1 0.0 2104 2 -5.751737020333849 2104 3 0.0 2104 4 -1.388181279620853 2104 5 -7.442391287962899 2104 6 -5.326614301731596 2104 7 -10.254291371857539 2104 8 -3.700947397189898 2104 9 1.4479413603285938 2104 10 4.5675438862487 2104 11 4.7612327260961465 2104 12 -16.794493234589503 2104 13 -5.918599641570793 2104 14 -5.327582620425698 2104 15 0.9058637837486856 2104 16 3.9093338810553497 2104 17 -17.522189126229833 2104 18 -8.034567543495953 2104 19 -5.266385016211409 2104 20 -5.107840232892741 2104 21 -19.36321448194163 2104 22 -9.236298247400466 2104 23 -19.932056401761045 2104 24 -10.087104036196944 2104 25 -5.314844759825043 2104 26 0.6015928781613553 2104 27 2.4772030167919503 2104 28 3.242188660324892 2104 29 3.3731020619579795 2104 30 2.397204762963277 2104 31 0.6163747492182949 2104 32 2.305049829152175 2104 33 4.333192238413158 2104 34 4.22669104173286 2104 35 2.224921203297523 2104 36 2.432324422246971 2104 37 -1.427739694760163 2104 38 1.0827395784422436 2104 39 1.8586328604021212 2104 40 -18.890407964464345 2104 41 -8.482295577931591 2104 42 -4.054745639557755 2104 43 -3.041422494069849 2104 44 -0.5362625878034137 2104 45 -0.11388096540962067 2104 46 0.29150497572474876 2104 47 0.14702959288960926 2104 48 -0.7465532306502602 2104 49 0.41235991156085205 2104 50 0.34862789469332967 2104 51 -0.12741121859681925 >>END_MODULE >>Per sequence quality scores fail #Quality Count 5 4.0 6 213.0 7 1730.0 8 6907.0 9 16931.0 10 30532.0 11 46938.0 12 64427.0 13 83463.0 14 104084.0 15 124739.0 16 144125.0 17 162197.0 18 176944.0 19 189359.0 20 195228.0 21 175869.0 22 158311.0 23 152927.0 24 153831.0 25 150554.0 26 134147.0 27 105586.0 28 56244.0 29 16278.0 30 3261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 NaN NaN NaN NaN 2 37.69170282365143 27.679430957325806 27.29283911350795 7.336027105514805 3 NaN NaN NaN NaN 4 38.44343736527025 22.62604122442397 28.33841809583049 10.592103314475281 5 35.281465085489536 27.402415080913073 26.996399422979493 10.319720410617899 6 13.093368790758909 33.732192922219646 40.878642878699736 12.295795408321707 7 21.58810915494083 30.52324448009511 31.695391370764398 16.193254994199666 8 21.339734358800968 32.42228029403438 29.600193904632327 16.63779144253233 9 22.06234056634689 31.644166575638216 29.780503633641548 16.51298922437335 10 22.512567127357507 29.53908303925817 29.29657774498226 18.651772088402062 11 22.43308183881385 30.736861565514424 29.130675219522523 17.699381376149205 12 21.93768846453581 29.941866384313087 30.077782981409463 18.042662169741643 13 21.923468341619685 29.409637375681204 29.89256899493413 18.774325287764977 14 21.62557962285764 30.616755790321854 29.218369181723048 18.539295405097462 15 21.09692953785524 30.12105023067264 29.514435263521744 19.267584967950377 16 21.568253291551223 29.796188879585706 29.719115687512886 18.91644214135019 17 21.640762575606768 31.25960417585522 28.520452783310386 18.579180465227626 18 21.098658197671625 30.598250418167495 29.394791665817976 18.908299718342906 19 21.299813551167922 30.066249013678753 29.380376392815954 19.25356104233737 20 21.547692323986723 30.538705547311036 29.191564870709936 18.72203725799231 21 20.6550652418177 30.282652840226497 29.879114288411596 19.183167629544204 22 21.307085863591535 29.962941825142607 29.204383757626417 19.525588553639437 23 21.317988378830147 30.25428482047162 29.176253792429396 19.25147300826884 24 20.773544715334552 30.203244299297427 29.95613951114314 19.067071474224882 25 21.41831467690825 30.099367410112883 29.24109988923872 19.241218023740146 26 21.23058165844393 30.717378593688206 29.087767696573863 18.964272051294 27 20.578827992145694 30.745951134420725 29.51464375289864 19.160577120534942 28 21.28468852378823 30.218040501345016 29.34665380030652 19.15061717456023 29 21.24935105089376 30.68725152459777 29.125528100361564 18.937869324146906 30 20.754561723036513 30.2080511514244 29.5082060705654 19.529181054973687 31 20.815706511532984 30.298525885102386 29.146470079993353 19.73929752337128 32 20.96260065365042 30.815181016681812 29.155472743722676 19.066745585945093 33 20.447243652634427 30.579124137848286 29.470982878333814 19.502649331183473 34 21.13560029737354 30.111352133044107 29.215728208731782 19.537319360850567 35 21.040526381825618 30.867941776017233 28.685438627079403 19.40609321507775 36 20.581118240146655 30.449292188613914 29.648314492310824 19.321275078928608 37 20.675678373085947 30.402838186464354 29.49775685013119 19.423726590318505 38 21.122131437042444 30.953776687468455 29.032364506955272 18.891727368533832 39 20.171873478763693 30.672441950131763 29.949458801407346 19.2062257696972 40 20.799218829540088 30.540710642984735 29.17676295548994 19.48330757198523 41 21.136828675235627 30.850010326584865 28.971142185463833 19.04201881271567 42 20.476904908651477 30.556099834245074 29.763824690029328 19.20317056707412 43 20.75306904355017 30.412240732781886 29.610816558067913 19.22387366560003 44 20.81138336593539 30.72912503038599 29.29270393688198 19.16678766679664 45 20.208837386985863 30.637605422719393 30.057351918424118 19.096205271870623 46 20.69120358709687 30.53514576867324 29.457979833193793 19.315670811036103 47 20.85317171888756 31.022321348362325 29.31751325871106 18.80699367403906 48 20.519325309235988 30.57813365399225 29.924004162902136 18.97853687386962 49 20.64033420959333 30.4442213403347 29.841023345330854 19.074421104741116 50 20.83621151323704 30.922947022018228 29.39812100978057 18.84272045496416 51 20.70153927589867 30.458850193129354 29.585040429120074 19.254570101851893 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 42.0 1 57.0 2 72.0 3 143.5 4 215.0 5 301.0 6 387.0 7 551.0 8 715.0 9 1139.0 10 1563.0 11 2389.0 12 3215.0 13 4774.0 14 6333.0 15 9357.0 16 12381.0 17 17676.5 18 22972.0 19 31226.5 20 39481.0 21 51639.5 22 63798.0 23 79321.0 24 94844.0 25 113281.0 26 151373.5 27 171029.0 28 188223.0 29 205417.0 30 218562.0 31 231707.0 32 239023.5 33 246340.0 34 245786.0 35 245232.0 36 235782.0 37 226332.0 38 211975.0 39 197618.0 40 179764.0 41 161910.0 42 143470.0 43 125030.0 44 108973.5 45 92917.0 46 78718.0 47 64519.0 48 53400.5 49 42282.0 50 34497.5 51 26713.0 52 21531.5 53 16350.0 54 12927.0 55 9504.0 56 7530.0 57 5556.0 58 4452.5 59 3349.0 60 2649.5 61 1950.0 62 1582.5 63 1215.0 64 998.0 65 781.0 66 633.0 67 485.0 68 410.5 69 336.0 70 276.5 71 217.0 72 180.5 73 144.0 74 107.5 75 56.5 76 42.0 77 31.5 78 21.0 79 16.0 80 11.0 81 8.5 82 6.0 83 6.5 84 7.0 85 4.0 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 100.0 2 49.17898558310986 3 100.0 4 87.62239650908474 5 36.80138209219461 6 62.74591835113566 7 36.26582544038709 8 5.703044896406226E-4 9 36.602916129799674 10 11.726519443920534 11 24.200708073759923 12 11.974153800529487 13 13.379384063003982 14 0.0 15 13.379302590934033 16 12.474270101909338 17 24.448423902438826 18 0.0028922584831774433 19 0.0 20 0.0 21 11.974153800529487 22 0.0016294413989732077 23 11.974153800529487 24 0.0 25 0.0 26 11.726682388060432 27 11.726437971850585 28 11.729452438438685 29 11.726437971850585 30 0.0 31 0.0 32 0.0 33 11.72656017995551 34 1.6294413989732074E-4 35 0.0013442891541528962 36 0.003218146762972085 37 8.961927694352642E-4 38 0.0029329945181517736 39 0.0 40 11.975131465368872 41 0.0 42 0.0 43 0.0030144665881004337 44 0.1238782823569381 45 0.19117421213453156 46 0.21019794046754375 47 0.23924273340424118 48 0.26629146062719644 49 0.26661734890699107 50 0.2774938702451373 51 0.26356214628391633 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2454829.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.51265346329797 #Duplication Level Percentage of deduplicated Percentage of total 1 99.00108882806398 97.52859956208248 2 0.7688512558217893 1.5148315465918676 3 0.11456122959702428 0.338571921348629 4 0.05099915329475443 0.20096247661791017 5 0.028095262049237093 0.13838694071085203 6 0.01918707227361575 0.11341016411195767 7 0.007321263939678416 0.050486599618201566 8 0.0032082661986343078 0.025284385299525904 9 0.001428215579520275 0.01266275758105477 >10 0.0052594531817483445 0.07680364603751283 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 2.0368017487165093E-4 0.0 0.0 5 2.851522448203113E-4 0.0 0.0 6 0.0011813450142555755 0.0 0.0 7 0.03609212698725655 0.0 0.0 8 0.0367031675118715 0.0 0.0 9 0.03812892873597305 0.0 0.0 10 0.044565222261917226 0.0 0.0 11 0.06358895059492942 0.0 0.0 12 0.07817245111573963 0.0 0.0 13 0.09059694178291033 0.0 4.0736034974330185E-5 14 0.09642219478423955 0.0 4.0736034974330185E-5 15 0.10131051898115917 0.0 4.0736034974330185E-5 16 0.10819490889182098 0.0 4.0736034974330185E-5 17 0.11487561862761113 0.0 4.0736034974330185E-5 18 0.12330797786729748 0.0 4.0736034974330185E-5 19 0.133980819030572 0.0 4.0736034974330185E-5 20 0.14029490445159318 0.0 4.0736034974330185E-5 21 0.1479125429917929 0.0 4.0736034974330185E-5 22 0.15548944549701832 0.0 4.0736034974330185E-5 23 0.16424769301649933 0.0 4.0736034974330185E-5 24 0.1730059405359803 0.0 4.0736034974330185E-5 25 0.1836787816992548 0.0 4.0736034974330185E-5 26 0.19427015079258067 0.0 4.0736034974330185E-5 27 0.20445415953616322 0.0 4.0736034974330185E-5 28 0.21370123947533617 0.0 4.0736034974330185E-5 29 0.22311126355440644 0.0 4.0736034974330185E-5 30 0.23199171917881042 0.0 4.0736034974330185E-5 31 0.2424608801672133 0.0 4.0736034974330185E-5 32 0.25162648803643756 0.0 4.0736034974330185E-5 33 0.2612401922903795 0.0 4.0736034974330185E-5 34 0.272279657768423 0.0 4.0736034974330185E-5 35 0.2853966610301573 0.0 4.0736034974330185E-5 36 0.29830998411702 0.0 4.0736034974330185E-5 37 0.31378967740726543 0.0 4.0736034974330185E-5 38 0.3293508427674596 0.0 4.0736034974330185E-5 39 0.3469080738413959 0.0 4.0736034974330185E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGGTG 560 0.0 18.425537 44 GGTCGCC 170 0.0 18.392694 44 ATCTCGG 580 0.0 18.327772 42 TCTCGGT 610 0.0 16.146372 43 GGTAGTG 555 3.8896612E-4 15.886921 4 TCGGTGG 735 0.0 14.676319 45 GTCGCCG 225 0.0 14.591239 45 GATCTCG 820 0.0 12.5781555 41 GGATCGA 325 0.0028811288 11.627131 4 CGTGTGC 220 0.0032633683 11.450961 4 TGGTCGC 270 7.2759576E-11 11.0015545 43 CGGGTCG 135 0.0019797015 10.494555 36 CCCGGGG 160 0.0022821655 10.316455 16 AGATCTC 1040 0.0 9.555155 40 AGTGTAG 905 0.0 8.8058605 35 GTGGTCG 270 9.69263E-7 8.684729 42 TAGATCT 1215 0.0 8.469038 39 AGATCGG 1880 1.8189894E-12 8.462137 7 GAGTGTA 945 0.0 8.4331255 34 GTAGATC 1155 0.0 8.133618 38 >>END_MODULE